AffiPLATE® Poly-L-lysine Coated, 96 well Solid plates (White PS)
AffiPLATE® Poly-L-lysine Coated plates are high-performance, 96-well microplates made from white polystyrene (PS). Each well surface is coated with Poly-L-lysine, a synthetic amino acid polymer that enhances cell attachment and adhesion. The white PS composition is optimized for luminescence and colorimetric applications, providing excellent signal reflectivity and sensitivity.
Key Applications:
Cell Culture and Adhesion:
- Cell Attachment: The Poly-L-lysine coating provides a positively charged surface that promotes the adhesion of negatively charged cell membranes. This is particularly useful for culturing anchorage-dependent cells, such as primary neurons, endothelial cells, and various adherent cell lines.
- Tissue Engineering: These plates support the growth and differentiation of stem cells and other sensitive cell types, making them ideal for tissue engineering applications. The enhanced adhesion properties help maintain cell morphology and function.
Molecular and Cellular Assays:
- Immunocytochemistry (ICC): AffiPLATE® Poly-L-lysine coated plates facilitate the adhesion of cells for subsequent staining and imaging in ICC assays. The stable attachment ensures consistent cell morphology and localization of cellular components.
- In Situ Hybridization (ISH): The coating supports the attachment of cells or tissue sections for ISH, allowing for the detection of specific nucleic acid sequences within intact cells. This is critical for studying gene expression and localization.
High-Throughput Screening (HTS):
- Drug Testing: The 96-well format allows for high-throughput drug screening on adherent cells. The Poly-L-lysine coating ensures uniform cell attachment across all wells, providing consistent assay conditions for reliable drug efficacy and toxicity testing.
- Genetic Screening: These plates are used in genetic screening assays where cell adhesion is crucial. The robust cell attachment facilitates the screening of gene function, RNA interference, and CRISPR-mediated gene editing.
Luminescence and Colorimetric Assays:
- Luminescent Assays: The white PS provides high reflectivity, enhancing the signal in luminescent assays. This is beneficial for applications such as ATP quantification, reporter gene assays, and luminescence-based cell viability assays.
- Colorimetric Assays: The plates are optimized for colorimetric detection, providing a clear and consistent background for assays such as ELISA, enzyme activity measurements, and protein quantification.
Neuroscience Research:
- Neuronal Cultures: The Poly-L-lysine coating is particularly advantageous for culturing primary neurons and other neural cells. It supports neurite outgrowth and synapse formation, making these plates ideal for neurophysiological and neuropharmacological studies.
- Neurotoxicity Testing: These plates are used in neurotoxicity assays to assess the effects of compounds on neuronal viability and function. The stable attachment of neurons ensures reliable and reproducible results.
Diagnostic Applications:
- Cell-Based Diagnostics: AffiPLATE® Poly-L-lysine coated plates are used in diagnostic assays that require the attachment of patient-derived cells. This includes assays for detecting infectious agents, cancer biomarkers, and other disease-related cellular changes.
- Biomarker Analysis: These plates facilitate the adhesion of cells for subsequent biomarker analysis using immunoassays or molecular techniques, supporting the identification and quantification of disease markers.
Technical Specifications:
- Material: White Polystyrene (PS)
- Well Count: 96 wells
- Surface Coating: Poly-L-lysine
- Well Volume: Typically 300 µL per well
- Compatibility: Compatible with standard microplate readers and robotic handling systems
- Storage Conditions: Store in a dry environment at room temperature, avoid exposure to direct sunlight
Handling and Usage:
- Preparation: Ensure plates are equilibrated to room temperature before use to maintain optimal cell attachment conditions.
- Washing: Gently wash wells with appropriate buffers to remove non-adherent cells and debris.
- Cell Seeding: Seed cells uniformly across wells to achieve consistent cell density and morphology.
By leveraging the Poly-L-lysine coated surface of AffiPLATE® Poly-L-lysine Coated, 96-well Solid Plates, researchers can achieve enhanced cell attachment and proliferation, enhancing the performance and reliability of various cell-based and molecular assays.