AffiPEG® mPEG-Biotin, 10K
AffiPEG® mPEG-Biotin, 10K is a high-purity, mono-functional polyethylene glycol (PEG) derivative specifically designed for biotinylation of biomolecules. With a molecular weight of 10,000 Daltons, this PEG derivative features a methoxy group (mPEG) on one end and a biotin group on the other. The biotin moiety enables strong and specific binding to streptavidin and avidin, making it an invaluable tool in various biotechnological and biomedical applications.
Applications of AffiPEG® mPEG-Biotin, 10K
Protein and Peptide Biotinylation:
Affinity Purification: AffiPEG® mPEG-Biotin, 10K is widely used to biotinylate proteins and peptides, facilitating their purification via streptavidin or avidin affinity chromatography. This method leverages the high affinity between biotin and streptavidin/avidin to isolate and purify biotinylated molecules.
Labeling and Detection: Biotinylated proteins and peptides can be easily detected and quantified using streptavidin or avidin conjugated to various reporter molecules (e.g., enzymes, fluorophores), making this product ideal for Western blotting, ELISA, and other detection assays.
Cell and Tissue Targeting:
Targeted Drug Delivery: The biotin-streptavidin interaction is employed to target drugs to specific cells or tissues. By biotinylating therapeutic agents with AffiPEG® mPEG-Biotin, 10K, they can be directed to target cells coated with streptavidin, enhancing the precision and efficacy of drug delivery systems.
Cell Surface Modification: Cells can be biotinylated using this product, enabling their subsequent capture or manipulation using streptavidin-coated surfaces or particles. This is useful in cell sorting, tracking, and targeting applications.
Bioconjugation and Crosslinking:
Stable Conjugates: The biotin end group of AffiPEG® mPEG-Biotin, 10K allows for stable and specific conjugation to streptavidin or avidin-tagged molecules. This bioconjugation is used in creating complex biomolecular assemblies and diagnostic reagents.
Crosslinking Biomolecules: The product facilitates the crosslinking of biotinylated biomolecules to streptavidin or avidin, forming stable complexes that can be used in various biochemical assays and structural studies.
Nanoparticle Functionalization:
Surface Modification: AffiPEG® mPEG-Biotin, 10K is used to functionalize the surface of nanoparticles, providing a biotin tag that enables subsequent binding to streptavidin or avidin. This enhances the targeting and delivery capabilities of nanoparticles in drug delivery and diagnostic applications.
Enhanced Stability: The PEG moiety improves the stability and solubility of nanoparticles, reducing aggregation and enhancing their biocompatibility and circulation time in vivo.
Tissue Engineering and Biomaterials:
Scaffold Functionalization: In tissue engineering, biotinylated scaffolds can be created using AffiPEG® mPEG-Biotin, 10K. These scaffolds can then be functionalized with streptavidin-linked growth factors or cell adhesion molecules, promoting cell attachment and tissue regeneration.
Hydrogel Formation: The product can be incorporated into hydrogels, providing sites for biotin-streptavidin interactions that enable the controlled attachment and release of biomolecules.
Diagnostic and Imaging Agents:
Enhanced Imaging: Biotinylation of imaging agents with AffiPEG® mPEG-Biotin, 10K allows for their subsequent binding to streptavidin-conjugated imaging probes, enhancing the specificity and quality of imaging.
Biosensor Development: The biotin-streptavidin system is commonly used in biosensor applications, where AffiPEG® mPEG-Biotin, 10K facilitates the attachment of biotinylated sensing elements to streptavidin-coated surfaces.
Technical Specifications
Molecular Weight: 10,000 Daltons
Functional Groups: Methoxy (mPEG) on one end, Biotin on the other end
Solubility: Highly soluble in water and most organic solvents
Purity: High purity with minimal polydispersity index
Handling and Storage
Storage Conditions: Store at -20°C in a dry and inert atmosphere to prevent degradation and contamination.
Handling Precautions: Use personal protective equipment (PPE) such as gloves and safety glasses when handling the product. Avoid inhalation and contact with skin or eyes.
AffiPEG® mPEG-Biotin, 10K is an essential tool for biotinylation and bioconjugation in a wide range of applications, including protein purification, targeted drug delivery, nanoparticle functionalization, tissue engineering, and diagnostic assay development. Its high specificity and biocompatibility make it an invaluable reagent in advanced biotechnological and biomedical research.