AffiGEN® 1st Strand cDNA Synthesis Kit
AffiGEN® 1st Strand cDNA Synthesis Kit (No Dye) is a rapid reverse transcription kit suitable for PCR amplification and RT-qPCR experiments. It has stable detection rates, specificity, and yield assurance, with the total reverse transcription time potentially reduced to less than 6 minutes, significantly shortening experimental duration.
The kit includes gDNA Digester Mix, which can eliminate genomic DNA contamination remaining in the RNA template, ensuring more reliable subsequent results. The kit provides two types of cDNA synthesis primers: Random Primers N6 and Oligo (dT)18. Users can choose between Random Primers N6, Oligo (dT)18, or Gene Specific Primers as reverse transcription primers according to their needs. The synthesized single-stranded cDNA product can be directly used for subsequent PCR or qPCR reactions.
Components:
- 4x PCR mastermix (No Dye) – 500 uL
- 5x gDNA Digester Mix – 200 uL
- Random Primers (50 μmol/L) – 200 uL
- Oligo d(T)18 Primers (50 μmol/L) – 200 uL
- RNase-free Water – 2x 1mL