SKU: AFG-CHK-0484

AffiCHECK® Echovirus 5 (EV5) RNA - PCR Qualitative Positive Control

Vendor AffiGEN
Regular price 6 203 SEK
Sale price 6 203 SEK Regular price
Sale Sold out
Unit price
/för 
Shipping calculated at checkout.
Size:
Pre Order text

AffiCHECK® Echovirus 5 (EV5) RNA - PCR Qualitative Positive Control

Product Datasheet - AFG-CHK-0484

PRODUCT DESCRIPTION:

AffiCHECK® Echovirus 5 (EV5) RNA - PCR Qualitative Control is a high-quality, reliable standard containing known quantities of Echovirus 5 (EV5) RNA. It's designed using modified and non-infectious parasite to ensure stability, thereby contributing to accurate and reliable results in PCR applications.

INTENDED USE:

AffiCHECK® Echovirus 5 (EV5) RNA - PCR Qualitative Control serves as a comprehensive process control for the evaluation of the performance of nucleic acid tests for determination of the presence of Echovirus 5 (EV5) RNA nucleic acid. It's essential in validating the performance of PCR assays in clinical, research, and diagnostic settings.

PRODUCT FEATURES:

  • Specificity: Tailored for Echovirus 5 (EV5) RNA detection.
  • Formulation: Lyophilized for enhanced stability.
  • Convenience: Ready-to-use upon reconstitution.
  • Compatibility: Suitable for a wide range of PCR assays.

CHARACTERISTICS:

  • Cultivation & Extraction: Cultivated in Vero cells and extracted using a reliable genomic RNA method.
  • Quality Control: Subjected to rigorous testing to ensure batch consistency and reliability.
  • Packaging: Provided in a protective, heat-sealed foil pouch with silica gel.

PERFORMANCE CHARACTERISTICS:

  • Sensitivity: Optimized for high sensitivity in PCR detection.
  • Specificity: Exclusively detects Echovirus 5 (EV5) RNA.
  • Reproducibility: Consistent results across different test runs and batches.

PACK CONTENT :

Components Pack Size Note 
RNA Control Lyophilized Powder (1.25x104 to 2.00x104 Copies/µL) 1 Vial Quantified by Real-Time PCR.
Buffer 500 µL Water, DNase, RNase Free.


PRECAUTIONS:

  • For research use or diagnostic control purposes only.
  • Follow standard laboratory practices and use PPE.
  • Use Sterile Filter Tips to prevent contamination.
  • Handle infectious specimens with precautions.
  • Disinfect work surfaces.
  • Maintain separate working areas for testing.
  • Dispose of unused reagents and waste as per regulations.
  • May contain genetic material, handle with care.
  • No method ensures total absence of infectious agents.
  • Follow local waste disposal rules.
  • Dilute below 103 copies/µL immediately before use.
  • Avoid freezing for low-copy dilutions to prevent RNA degradation.

INSTRUCTIONS FOR USE:

  • Open the foil pouch of AffiCHECK® Echovirus 5 (EV5) RNA.
  • Centrifuge AffiCHECK® Echovirus 5 (EV5) RNA Lyophilized Powder at 1000 g for 1 minute.
  • Add 100 μL of the Buffer and thoroughly mix until fully reconstituted.
  • The concentration will be 1.25x104 to 2.00x104 Copies/µL once reconstituted.
  • Vortex for 1 min for complete dissolution and homogenization.

RECOMMENDED STORAGE:

  • Before Reconstitution: Store at 2-8ºC.
  • After Reconstitution: Store at -20ºC.
  • Shipping: No special instructions
  • Shelf Life: 12 months from production date.

ORDERING INFORMATION :

Catalog Number Description Size
AFG-CHK-0484 AffiCHECK® Echovirus 5 (EV5) RNA PCR Qualitative Positive Control 1 vial + 500 µL Buffer