AffiVET® Swine PRRS Antibody ELISA Kit
Species: Swine
Disease or Pathogen: Porcine Reproductive and Respiratory Syndrome Virus
Target: Antibody
Test Type: Elisa Kit
Porcine Reproductive and Respiratory Syndrome antibody
Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failure of sows and respiratory problems of piglets and growing pigs. The disease is caused by PRRS virus, currently classified as a member of family Arteriviridae, genus Arterivirus. The primary target cell of the virus is alveolar macrophage of pigs. Two major types of the virus exist, the European (EU) and the North America (NA) strain. The virus is primarily transmitted by contaminated feces, urine, semen and infected pigs. High level of sanitary management system is required because fomite infection is also possible.
Indications:
- Routine monitoring of PRRS
- PRRS diagnosis of non-vaccinated group
- Titer check after vaccination
Special Features:
- No singleton reactor (false positive)
- Antibodies against European strain, North America strain and Korean strain can be detected
- No cross reaction with other swine disease positive sera
- Specimen: Plasma, Serum
- Reading time: 75 minutes
- Sensitivity: 98.7% vs. IFA
- Specificity: 99.7% vs. IFA
Quick Procedure:
- Prepara antigen coated micro assay plate
- Dilute test samples and controls with sample diluent (1:39 dilution)
- Add 100 μl of diluted samples and controls to wells
- Incubate plate for 30 minutes at room temperature
- Wash plate 5 times
- Add 100 μl of enzyme conjugate to wells
- Incubate plate for 30 minutes at room temperature
- Wash plate 5 times
- Add 100 μl of substrate solution and incubate for 15 minutes at room temperature in dark
- Add 100 μl of stop solution
- Measure the optical density (OD) at 450 nm with reference wavelength at 620 nm
- S/P value=[OD sample-mean OD NC]/[ mean OD PC- mean OD NC]
Sensitivity and specificity study
-AffiGen PRRS Ab ELISA 4.0 has high sensitivity, 98.7% and high Specificity, 99.7%