AffiPURE® Bacteria DNA Isolation Mini Kit
AffiPURE® Bacteria DNA Isolation Mini Kit is specially designed for genomic DNA isolation from bacteria of various origins (Gram-negative bacteria, Gram-positive bacteria). The kit uses silica gel membrane purification technology. No toxic reagents such as phenol chloroform are needed during the extraction process, and no time-consuming alcohol precipitation is required. It minimizes RNA, impurities, lipids and other inhibitory impurities to the utmost extent. The extracted genomic DNA has high purity and stable quality, and can be used in downstream experiments such as digestion, PCR, and Southern hybridization.
Components:
| Components | AFG-VZ-DC103-01 |
| Buffer GA | 40 mL |
| Proteinase K | 44 mg |
| Proteinase K Dissolve Solution | 4 mL |
| RNase A | 400 uL |
| Buffer GB | 40 mL |
| Buffer PB | 26 mL |
| Buffer PW | 40 mL |
| Elution Buffer | 40 mL |
| AffiPURE gDNA Mini Columns III | 100 |
| Collection Tubes 2 ml | 100 |
Note:
Storage:
Store RNase A and Proteinase K dry powder at -30°C ~ -15°C. and transport at room temperature. The other components should be stored at room temperature (15°C - 25°C) and transported at room temperature.
Applications:
Genomic DNA isolation from < 1.0 x 109 Gram-negative bacteria or Gram-positive bacteria.
Additional Materials Required:
Lysozyme (AffiGEN, Cat.# AFG-VZ-DE103) (for Gram-positive bacteria), absolute ethanol, 1.5 ml centrifuge tube, water bath or metal bath.