AffiPCR® Universal SYBR qPCR Master Mix
AffiPCR® Universal SYBR qPCR Master Mix is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component, Champagne Taq DNA Polymerase, is a novel hot-start DNA polymerase modified by antibody method. It has many advantages such as strong specificity, high detection sensitivity. With the optimal Buffer optimized for qPCR and the specific promoter, it is very suitable for qPCR reaction with high specificity and sensitivity. This kit contains special ROX, Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments, and the amplification can be performed with the addition of primers and templates when preparing the reaction system.
Storage Conditions:
Store at -30°C ~ -15°C and protect from light. Master mix can be stored stably at 2°C ~ 8°C for 6 months and protected from light after thawing. Transport at ≤ 0°C.
Application:
This kit is used for the amplification and quantification of DNA samples, which can amplify DNA from all species, including genomic DNA, cDNA, plasmid DNA, λDNA, etc.
Equivalence:
AFG-VZ-Q711 | AffiGEN AffiPCR® Universal SYBR qPCR Master Mix |
M3003S | NEB Luna®Universal qPCR Master Mix |
RR420 Q/A/B | TaKaRa TB Green®Premix Ex Taq™ (Tli RNaseH Plus) |
4913850001 | Roche FastStart Univrsal SYBR Green Master |