AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V3
AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V3 is an upgraded version of HiScript II Q RT SuperMix for qPCR (+gDNA wiper), including HiScript III Reverse Transcriptase, a new generation of reverse transcriptase with optimized Buffer. This kit further improves the efficiency of cDNA synthesis, and is suitable for two-step qRT-PCR detection. The 4X gDNA wiper Mix in the kit can remove residual genomic DNA from the RNA template, ensuring more reliable quantitative results. It simplifies qPCR primer design without the need of spanning an exon-exon junction; 5X HiScript III qRT SuperMix contains all the components required for the reverse transcription reaction. The reaction can be performed quickly by adding template RNA and RNase-free ddH2O, and at the same time, the gDNA wiper function is terminated to ensure the integrity of the cDNA. It is compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
Components:
| Components | Quantity |
| RNase-free ddH2O | 2 × 1 mL |
| 4 × gDNA wiper Mix | 400 μL |
| 5 × HiScript III qRT SuperMixa | 400 μL |
| 5 × No RT Control Mixb | 40 μL |
Application:
It is applicable for reverse transcription reactions of animal, plant and microbial RNA. The RT product is compatible with dye-based and probe-based qPCR.
Storage Conditions:
Store at temperatures between -30°C and -15°C, and ensure transportation at ≤0°C. After thawing, they can be stored at 4°C for up to three months, maintaining their quality and efficacy.
Equivalence:
| AFG-VZ-R323 | AffiGEN AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V3 |
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