AffiPCR® Max Super-Fidelity DNA Polymerase
Our AffiPCR® Max Super-Fidelity DNA Polymerase is perfect for PCR amplification. It is highly active and provides accurate and efficient replication with low background and high yield. It also has a 5’ to 3’ exonuclease activity which ensures faithful copying.
AffiPCR® Max Super-Fidelity DNA Polymerase is an upgraded of Max Super-Fidelity DNA Polymerase. Compared with the previous generation, AffiPCR® Max has added the unique elongation factor, specificity-enhancing factor and plateau phase anti-inhibitor factor, which greatly improves the long-fragment amplification ability, amplification specificity and amplification yield. AffiPCR® Max can efficiently amplify up to 40 kb simple templates (e.g. λDNA, plasmids), 20 kb complex templates (e.g. genomic DNA) and 10 kb cDNA. The amplification error rate of AffiPCR® Max is 128-fold lower than that of conventional Taq DNA Polymerase. In addition, AffiPCR® Max has a good resistance to PCR inhibitors and can be used for direct PCR amplification of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. AffiPCR® Max contains two monoclonal antibodies inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. Amplification products are blunt-ended, which are compatible with ClonExpress kits and TOPO cloning kit.
Components:
Components | 100 U | 500 U | 1,000 U |
DNA Polymerase | 100 μl | ||
2X Max Buffer | 2 × 1.25 ml | X 5 | X 10 |
dNTP Mix (10 mM each) | 100 μl | ||
10X DNA Loading buffer | 1.25 ml |
Equivalence:
AFG-VZ-P505 | AffiGEN AffiPCR® Max Super-Fidelity DNA Polymerase |
AFG-VZ-515 | AffiGEN 2X AffiPCR® Max Master Mix |
AFG-VZ-525 | AffiGEN 2X AffiPCR® Max Master Mix (Dye Plus) |
F537S | Thermo Fisher Phusion Green Hot Start II High-Fidelity DNA Polymerase |
KOD-401 | TOYOBO KOD-Plus-Neo |
KFX-201 | TOYOBO KOD FX Neo |
M0493S | NEB Q5® Hot Start High-Fidelity DNA Polymerase |
M0494S | NEB Q5® Hot Start High-Fidelity 2X Master Mix |
R050A | TaKaRa PrimerSTAR® GXL DNA Polymerase |
R045A | TaKaRa PrimerSTAR® Max DNA Polymerase |
RR371A | Takara Ex Premier Polymerase Dye plus |
12351010 | Invitrogen Platinum™ SuperFi™ DNA Polymerase |
12344024 | Invitrogen AccuPrime™ Pfx DNA Polymerase |