AffiPCR® Bst II DNA Polymerase Large Fragment

AffiPCR® Bst II DNA Polymerase Large Fragment

AffiPCR® Bst II DNA Polymerase Large Fragment is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Compared with the previous generation, Bst II DNA Polymerase Large Fragment has superior amplification speed, dUTP tolerance, salt tolerance and thermal stability.

Storage Conditions:

Store at -30°C ~ -15°C and transport at ≤0°C.

Applications:

It is applicable for isothermal amplifications such as LAMP, HDA and RCA.

Source:

Bst II Pro DNA Polymerase Large Fragment comes from Bacillus stearothermophilus.

Unit Definition:

One unit (U) is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid insoluble material in 30 min at 65°C.

Self-prepared Materials:

Reagents: dNTP Mix, FIP/BIP Primers, F3/B3 Primers, LoopF/LoopB Primers, Nuclease-free ddH2O. Instruments: qPCR instrument, PCR instrument or water bath.

Notes:

For research use only. Not for use in diagnostic procedures.