AffiNGS® TruePrep DNA Library Prep Kit V2 for Illumina
AffiNGS® TruePrep DNA Library Prep Kit V2 for Illumina is specifically designed for next-generation sequencing (NGS) on Illumina® platforms. This Kit enables super fast and easy preparation of ready-to-use DNA library for sequencing by adopting a new-type transposase. which convert the complex steps of DNA fragmentation. End repair dA-tailing. and adapter ligation into a one-step enzymatic reaction. significantly reducing the demanded amount of the initial DNA and shortening the time of library preparation. Three kinds of specifications of this kit are provided for different amount of input DNA: 50 ng(#TD501), 5 ng (#AFG-VZ-TD502), and 1 ng (#AFG-VZ-TD503).
Comtents:
|
Cat.No. Size InputDNA |
AFG-VZ-TD501-01/02 24/96 rxn 50 ng |
AFG-VZ-TD502-01/02 24/96 rxn 5 ng |
AFG-VZ-TD503-01/02 24/96 rxn 1 ng |
| TTE Mix V50 | 120/480 µl | ---- | ---- |
| TTE Mix V5 | ----- | 120/480 µl | ---- |
| TTE Mix V1 | ----- | 120/480 µl | |
| 5×TTBL | 240/960 µl | 96/384 µl | 96/384 µl |
| 5×TS | ----- | 120/480 µl | 120/480 µl |
| PPM | 120/480 µl | ---- | ---- |
| TAE | 24/96 µl | 24/96 µl | 24/96 µl |
| 5×TAB | 240/960 µl | 240/960 µl | 240/960 µl |
| ControlDNA | 10/10 µl | 10/10 µl | 10/10 µl |
TTE = TruePrep Tagment Enzyme; TTBL = TruePrep Tagment Buffer L; TS = Terminate Solution; PPM = PCR Primer Mix; TAE = TruePrep Amplify Enzyme; TAB = TruePrep Amplify Buffer.
*Control DNA, Mouse Genomic DNA, 50 ng/μl.
Storage:
AFG-VZ-TD501: store at -20℃.
AFG-VZ-TD502: store 5× TS at room temperature and other components at -20℃.
AFG-VZ-TD503: store 5× TS at room temperature and other components at -20℃.