AffiGEN® TransfectTM siRNA In Vitro 3000
Revolutionary Liposome-Based RNA Delivery System
AffiGEN® TransfectTM siRNA In Vitro 3000 is at the forefront of RNA delivery technology, offering an advanced liposome formulation designed to optimize in vitro RNA transfection. It represents a leap forward in the field, providing researchers with an exceptionally powerful tool for their experiments.
Kit Composition and Volume:
AffiGEN® TransfectTM siRNA In Vitro 3000: Comes in a 1.0 ml volume, enough to perform approximately 1330 transfections, assuming the use of 5.0 pmol siRNA per reaction in a 24-well plate format.
AffiGEN® TransfectTM siRNA In Vitro 3000 Buffer: An 8.0 ml buffer, engineered to enhance transfection efficiency to its maximum potential.
Storage Guidelines:
AffiGEN® TransfectTM siRNA In Vitro 3000: Should be stored at a constant 4 °C.
AffiGEN® TransfectTM siRNA In Vitro 3000 Buffer: Keep at room temperature (RT) and note it's provided in a 5x concentrate form.
Adherence to these storage conditions ensures the kit remains effective for 12 months or even longer, maintaining its stability and efficacy.
Distinguished Advantages:
The AffiGEN® TransfectTM siRNA In Vitro 3000 distinguishes itself through its cutting-edge lipid-conjugation technology and proprietary formulation, setting a new standard in the RNA delivery domain:
- Unmatched Efficiency: Engineered to deliver siRNA with exceptional efficiency across a wide array of mammalian cell types, including those that are traditionally challenging to transfect. It achieves this with remarkably low cytotoxicity, safeguarding cell viability.
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Cost Efficiency: Offers a more economical option without compromising on quality or effectiveness, making high-quality transfection accessible to more researchers.
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Additional Benefits: This reagent excels in various aspects that enhance its usability and effectiveness:
- Resistance to sera interference, ensuring consistent and reliable transfection outcomes.
- High stability for dependable performance over time.
- A straightforward and rapid procedure, facilitating ease of use.
- Ideal for high-throughput applications, allowing for scalable research projects.
Elevate your research with AffiGEN® TransfectTM siRNA In Vitro 3000 — the epitome of efficiency and innovation in RNA delivery.
siRNA In Vitro Transfection Reagents Specifications and Guide Application
| Product | AffiGEN® TransfectTM siRNA In Vitro 1000 | AffiGEN® TransfectTM siRNA In Vitro 2000 | AffiGEN® TransfectTM siRNA In Vitro 3000 | AffiGEN® TransfectTM siRNA In Vitro 4000 | AffiGEN® TransfectTM siRNA In Vitro 1000 Plus |
| Nucleic Acid | siRNA | siRNA, miRNA, DNA | siRNA | siRNA | siRNA |
| Technology | Peptide-based | Non-liposomal | Liposome formulation | Polymer-based | Peptide-based |
| Efficiency | High | High | High | High | High |
| Cell compatibility | Wide | Wide | Wide | Wide | Wide |
| Off-target effects | Low | Low | Low | Low | Low |
| Cytotoxicity | Moderate | Moderate | Low | Very Low | Moderate |
| Package sizes | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL |
| Storage | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT |
| Applications | Gene silencing, RNAi | Gene silencing, miRNA delivery, DNA delivery | Gene silencing | Gene silencing | Gene silencing |
| Target Organisms | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells |
| Cell Types Suitable For | Wide range of cell types | Wide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells | Wide range of cell types | Wide range of cell types, including suspension cells and difficult-to-transfect cells | Wide range of cell types, including hard-to-transfect cells |
| Additional Notes | N/A | Can be used for in vivo applications | N/A | Optimized for siRNA delivery, but can also be used for DNA transfection | Effective at low concentrations |