AffiGEN® Express MultiS Fast Mutagenesis Kit V2

AffiGEN® Express MultiS Fast Mutagenesis Kit V2

AffiGEN® Express MultiS Fast Mutagenesis Kit V2 is a kit based on ClonExpress rapid cloning technology, which can simultaneously introduce site-directed mutagenesis to 3 - 5 discontinuous sites on the target plasmid. The kit integrates the Phanta Max Super-Fidelity DNA Polymerase amplification system and the ClonExpress rapid cloning system. With superior fidelity, Phanta Max significantly reduces the possibility of introducing novel mutations during amplification. Its excellent long fragment amplification ability is widely applicable to the amplification of any plasmid less than 20 kb. The ClonExpress rapid cloning system replaces the conventional annealing-cyclization reactions with the more efficient homologous recombination reaction. Site-directed mutagenesis using the Mut Express MultiS Fast Mutagenesis Kit V2 allows for more flexible primer design and extremely low template input, thereby facilitating complete degradation of the initially methylated template. The kit is equipped with the highly optimized recombination reaction buffer and the recombinase Exnase MultiS, which specially optimized for multi-base site-directed mutagenesis. If the amplification product is highly specific, its Dpn I-digested product can be used directly in the recombination reaction without DNA purification. This product is the preferred kit for DNA multiple point mutation.

Application:

• Mutation: 3 - 5 discontinuous mutation sites (multiple bases within 50 bp apart can be counted as 1 mutation site).
• Deletion: delete a specific sequence on the vector.