5min Universal Ligation Mix is a ready-to-use 2 × premix solution, containing T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA or RNA. This kit is suitable for sticky end ligation, blunt end DNA ligation and TA ligation. Buffer with optimized ligation enhancer make the reaction more efficient and convenient. Ligation can be completed in 5 min at 25℃. The product can be used directly to transform many chemically competent cells.
Storage Conditions:
Store at temperatures between -30℃ and -15℃, and ensure transportation at ≤0℃. After thawing, they can be stored at 4℃ for up to three months, maintaining their quality and efficacy.