AffiAB® dsRNA 142 bp – Positive Control for dsRNA Detection
Description
The AffiAB® dsRNA 142 bp is a synthetic, chemically defined double-stranded RNA consisting of 142 base pairs. Designed as a positive control in dsRNA detection applications, it is free from viral or transforming sequences, ensuring biosafety and experimental consistency. It serves as a high-quality reference standard for use in ELISA, dot blot, RNAi analysis, and gel-based detection assays.
Key Features
- Defined 142 bp length, suitable as a size standard
- Sequence is non-viral and non-transforming
- Compatible with non-denaturing PAGE and agarose gels
- Ideal for use in ELISA, dot blot, and RNAi pathway validation
Formulation
- Form: Lyophilised from a 1 mg/mL solution in STE buffer
- Solubility: Reconstitute in 50 μL RNase-free H₂O to obtain a 1 μg/μL solution
Storage and Handling
- Shipping: Shipped on gel packs
- Storage:
- Upon receipt: store lyophilised at -20 °C to -80 °C
- After reconstitution: store in aliquots at -20 °C to -80 °C
- Avoid freeze/thaw cycles
- Shelf life: 12 months from delivery date
Quality Control
- Purity: Confirmed by absorbance spectrum and gel electrophoresis
- Activity: Validated via sandwich ELISA (relative to internal reference antigen)