AffiNGS® VAHTS Universal DNA Library Prep Kit for Illumina V4
AffiNGS® VAHTS Universal DNA Library Prep Kit for Illumina V4 is a library preparation kit specifically optimized for the Illumina high-throughput sequencing platform. It can convert 100 pg - 1 μg Input DNA into a specialized Illumina library. As an upgraded version, the kit features an increased conversion rate of low-quality template library and a lower library duplication rate with optimized end-repair module, ligation module and library amplification module. The kit is widely applicable to PCR or PCR-free library construction from multiple sample types, and compatible with the targeted capture process. All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.
Components:
| Components |
AFG-VZ-ND610-01 (24 rxns) |
AFG-VZ-ND610-02 (96 rxns) |
AFG-VZ-ND610-03 (24 rxns) |
AFG-VZ-ND610-04 (96 rxns) |
| End Prep Buffer 2 | 168 µl | 672 µl | 168 µl | 672 µl |
| End Prep Enzyme 2 | 72 µl | 288 µl | 72 µl | 288 µl |
| Rapid Ligation Buffer 5 | 720 µl | 4 X 720 µl | 720 µl | 4 X 720 µl |
| Rapid DNA Ligase 2 | 240 µl | 4 X 240 µl | 240 µl | 4 X 240 µl |
| VAHTS HiFi Amplification Mix 2 | 600 µl | 4 x 600 µl | / | / |
| PCR Primer Mix 3 for Illumina | 120 µl | 480 µl | / | / |
| Control DNA (264 bp, 50 ng/μl) | 10 µl | 10 µl | / | / |
Storage:
Store at -30 ~ -15℃ and transport at ≤0℃.