AffiVET® Peste Des Petits Ruminants Virus (PPRV) Antibody Rapid Test

AffiVET® Peste des Petits Ruminants Virus (PPRV) Antibody Rapid Test

Product Insert

Test Principal:

This test device uses the colloidal gold immunochromatography technique to qualitatively detect the Peste des Petits Ruminants Virus (PPRV) Antibody in serum or whole blood from goats and sheeps.

In the testing process, PPRV antibodies in the sample bind with the antigens that are coated on the colloidal gold, forming complexes.

These complexes then move towards the other edge of the card. When they reach the T-line, specific antigens on the membrane capture the complexes and the T-line appears.

The presence of a C-line indicates that the test is valid, and the appearance of a T-line indicates the presence of PPRV antibodies in the sample.

Material Required:

Storage & Stability:

• Store the test kit at 2 to 30°C. DO NOT FREEZE.
• Do not store the test kit in the direct sunlight.
• The test kit is stable within the expiration date marked on the package label.

Sample collection & Preparation:

Whole blood:

• Collect anticoagulated whole blood.
• Test the same day.

Serum:

• Collect blood and keep at 37℃ for 1-2h.
• Take the supernatant and centrifuge at 1500r/min for 10min then separate the serum.
• The serum can be stored for 2-3 days at 4℃.
• For long term storage, freeze at -20°C.

Test Procedure:

• Read the manual carefully.
• The test card and the sample need to be at room temperature before use.
• Take out the test card from the bag.
• Use the dropper to absorb the sample.
• Drop 2 to 3 drops into the well marked with “S”.
• Read the results in 5 to 10 min at room temperature.
•  The result is invalid after 15 min.

Result Interpretation:

Negative result

This means that there is no PPRV Antibodies in the sample or the leverl is lower than the test detection limit.

Positive result:

This confirms the presence of the PPRV antibodies in the sample.

Invalid Result:

Absence of C-Line
Presence of T-Line

Or

Absence of C-Line
Absence of T-Line

This means that the procedure was not applied correctly or that the test is invalid.

Cautions:

• The test card can only be used when brought to room temperature.
• Don’t reuse the test card or use it after the expiry date.
• Bring the sample and all the reagents to room temperature before use.
• When the the test card is unsealed, please use it as soon as possible.
• Take precaution while preparing the sample. Gloves and mask are required.
• The whole blood needs to be collected in anticoagulant tube.
• Don’t freeze the whole blood.
• The serum sample can be stored frozen but avoid repeated freezing and thawing.
• This product is only for in vitro rapid diagnostic veterinary use.
• This a qualitative test. It is not quantitative.
• The test resultas are for reference only. For confirmation, please proceed with your relevant national standard methods.