AffiPCR® Blood Direct PCR Kit V2
The AffiPCR® Blood Direct PCR Kit V2 offers reliable, accurate PCR results in just a few hours. This kit is designed for rapid and direct PCR from an extracted sample of blood, eliminating the need for unnecessary processing steps. The kit is simple to use, and no other reagents are needed for the PCR reaction.
The AffiPCR® Blood Direct PCR Kit V2 can perform PCR directly on whole blood samples without the need for DNA purification or sample pretreatment. This kit is applicable for fresh blood, blood stored at 4°C, frozen blood, and dried blood stains stored on Whatman903 or FTA commercial cards, and is compatible with all conventional anticoagulants (EDTA, Citrate Acid, Heparin, etc.). It contains Phanta Blood Super-Fidelity DNA Polymerase, which is modified based on Phanta Super-Fidelity DNA Polymerase. This enzyme has superior resistance to PCR inhibitors in whole blood samples and can amplify whole blood concentrations up to 40%. Equipped with optimized 2X Phanta Blood Buffer V2, Blood Direct PCR Kit V2 can efficiently amplify genomic fragments up to 10 kb from whole blood samples. Phanta Blood Super-Fidelity DNA Polymerase produces blunt-ended products, which are applicable to ClonExpress and TOPO cloning kits . This kit provides the positive control primer mix that is compatible with mammals and other vertebrates, which can be used to perform positive control reactions.
Components:
| Components | 50 rxns (50 µl/rxn) | 200 rxns (50 µl/rxn) |
| Phanta Blood Super-Fidelity DNA Polymerase (1 U/μl) | 75 μL | 300 μL |
| 2X Phanta Blood Buffer V2 | 1.25 mL | 4 × 1.25 mL |
| Positive control primer mix (10 μM each) | 50 μL | 50 μL |
| 10 mM each dNTPs | 50 μL | 200 μL |
| 10X DNA Loading buffer | 1.25 mL | 1.25 mL |
Storage Conditions:
Store at temperatures between -30°C and -15°C, and ensure transportation at ≤0°C. After thawing, they can be stored at 4°C for up to three months, maintaining their quality and efficacy.
Equivalence:
| AFG-VZ-PD103 | AffiGEN AffiPCR® Blood Direct PCR Kit V2 |
| M0332S/L | NEB Hemo KlenTaq® |