AffiGEL® Matrix High Concentration, LDEV-Free
- Equivalent: 354248 | Corning® Matrigel® Basement Membrane Matrix High Concentration (HC), LDEV-free
- Matrix type: High concentration (≥18mg/ml)
- Application: Experiments such as angiogenesis, gel embolization, and in vivo tumor formation (for angiogenesis,it is recommended that the final concentration of AffiGEL® basement membrane matrix should be ≥10mg/ml)
Product Information
AffiGEL® Matrix High Concentration, LDEV-Free is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Source
Engelbreth-Holm-Swarm Mouse Tumor
Quality
- Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
- Extensive PCR testing is performed to screen for a number of pathogens
- Tested and found negative for bacteria, fungi, and mycoplasma
- Protein concentrations determined by Lowry method
- Endotoxin units measured by Limulus Amoebocyte Lysate assay
- Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
- Biological activity is determined for each lot using a neurite outgrowth assay
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws.
Application
- Cell Growth and Differentiation
- Metabolism/Toxicology Studies
- Invasion Assays
- in vitro and in vivo Angiogenesis Assays
- in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice