AffiDETECT® MycoBlue Mycoplasma Detector
AffiDETECT® MycoBlue Mycoplasma Detector is designed for rapid detection of mycoplasma contamination in cell culture. Its advantage is convenience, after adding 1 μl of the cell culture supernatant to the reaction system and incubating at 60℃ for 1 h, the results can be determined by visual observation. Detection can be easily completed in cell culture lab without performing PCR, qPCR, or electrophoresis. Compared with conventional PCR methods, MycoBlue Mycoplasma Detector is more resistant to the inhibitor in the culture supernatant, avoiding weak positive and false negative. There is no need to perform electrophoresis, avoiding false positive from aerosol contamination. The result is highly consistent with the most sensitive and accurate qPCR method. It has been validated that MycoBlue Mycoplasma Detector could detect up to 28 kinds of mycoplasma, including 8 commonly found strains in cell culture. This product is suitable for mycoplasma detection in various types of suspension and adherent cells, including CHO, Vero, hybridoma, Sf9, HEK293, etc. It has a wide range of cell culture medium compatibility. MycoBlue Mycoplasma Detector is applicable for routine mycoplasma detection in biopharmaceutical companies, vaccine/monoclonal antibody manufacturers, cell therapy/embryo laboratories, and other scientific research laboratories.
Storage Conditions:
Store at -30 ~ -15℃ and transport at ≤0℃.
Self-prepared Material:
PCR instrument or water bath.
Applications:
It is applicable for various types of suspension and adherent cells with a wide range of cell culture medium and serum compatibility, which include but do not limit to: Suspension cells: CHO, NS0, 293F, mouse hybridomas, Sf9, BHK21, etc. Adherent cells: Vero, MDCK, SP2/0, 293T, HepG2. HeLa, A549, MB-MDA231, L929, MEF, etc. Cell culture medium: CD FortiCHO, CDM4, Expi 293 Medium, CD Hybridoma, Grace, DMEM, 1640, F12, etc. Serum: Fetal bovine/calf serum, horse serum, Gibco KSR serum replacement, etc
Notes:
For research use only. Not for use in diagnostic procedures. Tighten the cap of the tube during storage to keep it sealed.