AffiAB® dsRNA 142 bp – Positive Control for dsRNA Detection

AffiAB® dsRNA 142 bp – Positive Control for dsRNA Detection

Description
The AffiAB® dsRNA 142 bp is a synthetic, chemically defined double-stranded RNA consisting of 142 base pairs. Designed as a positive control in dsRNA detection applications, it is free from viral or transforming sequences, ensuring biosafety and experimental consistency. It serves as a high-quality reference standard for use in ELISA, dot blot, RNAi analysis, and gel-based detection assays.

Key Features

• Defined 142 bp length, suitable as a size standard
• Sequence is non-viral and non-transforming
• Compatible with non-denaturing PAGE and agarose gels
• Ideal for use in ELISA, dot blot, and RNAi pathway validation

Formulation

• Form: Lyophilised from a 1 mg/mL solution in STE buffer
• Solubility: Reconstitute in 50 μL RNase-free H₂O to obtain a 1 μg/μL solution

Storage and Handling

• Shipping: Shipped on gel packs
• Storage:

1. Upon receipt: store lyophilised at -20 °C to -80 °C
2. After reconstitution: store in aliquots at -20 °C to -80 °C
3. Avoid freeze/thaw cycles

• Shelf life: 12 months from delivery date

Quality Control

• Purity: Confirmed by absorbance spectrum and gel electrophoresis
• Activity: Validated via sandwich ELISA (relative to internal reference antigen)