AffiNGS® VAHTS AmpSeq Library Prep Kit V3
AffiNGS® VAHTS AmpSeq Library Prep Kit V3 is based on ultra-multiplex PCR and several core technologies such as end primer digestion, used to construct amplicon libraries. The whole library construction workflow can be finished in one tube.The whole process takes less than 5 , of which the manual operation time is less than 1.5 h. It is compatible with genomic DNA, FFPE DNA, cfDNA, etc, and amount of Input DNA is 1 - 100 ng. This kit is intended for both the Illumina and lon Torrent sequencing platforms. And corresponding adapters (AffiNGS #AFG-VZ-NA111/NA121) are also provided. It is compatible with panels including lon AmpliSeq Séries Panel and AmpliSeq for Illumina Series Panel. as well as the corresponding individually customized panels.
This kit is comprised of multiplex amplification module, digestion module and ligation module, which has great amplification performance for different input amounts and customized panels, great efficiency of library ligation, and high coverage and high quality library quickly and easily, and get more stabel and reliable sequencing data. All the reagents provided in the kit have undergone rigorous quality control and functional testing. to ensure the optimal stability and repeatability of library construction.
Component:
| Components | AFG-VZ-N210-01 (24 rxns) | AFG-VZ-NA210-02 (96 rxns) |
| 4xX VAHTS Multi-PCR Mix | 120 µl | 480 µl |
| VAHTS Digest Mix 2 | 60 µl | 240 µl |
| VAHTS Ligation Enhancer | 144 µl | 576 µl |
| VAHTS Ligation Enzyme Mix 2 | 24 µl | 96 µl |
| VAHTS HIFI Amplification Mix | 600 µl | 4 x 600 µl |
| PCR Primer Mix for ION Torrent | 120 µl | 480 µl |
| PCR Primer Mix for lllumina | 120 µl | 480 µl |
| TE | 1 ml | 4 x 1 ml |
Storage:
Store at -30 ~ -15°C and transport at ≤0°C.