AffiNGS® TruePrep Flexible DNA Library Prep Kit for Illumina
The AffiNGS® TruePrep Flexible DNA Library Prep Kit for Illumina is a dedicated reagent kit developed for the Illumina high-throughput sequencing platform. This kit employs a novel transposase-based method for DNA fragmentation, simplifying the intricate steps of DNA fragmentation, end repair, and adapter ligation into a single enzymatic reaction, significantly reducing library construction time. Compared to conventional transposase library construction kits, this kit enhances compatibility with DNA template input by chemically coupling the transposase to magnetic beads. This advancement allows the preparation of Illumina high-throughput sequencing platform-specific sequencing libraries from 100 pg to 500 ng DNA templates. All reagents in the kit undergo rigorous quality control and functional validation, ensuring the stability and reproducibility of library construction.