AffiVET® Swine PED IgA Ab ELISA Kit
Species: Swine
Disease or Pathogen: Porcine Epidemic Diarrhea Virus
Target: Antibody
Test Type: Elisa Kit
Porcine Epidemic Diarrhea virus
Porcine epidemic diarrhea virus (PEDV) is a member of the family Coronaviridae. PEDV causes acute enteritis in swine of all ages, and it is often fatal for neonatal piglets.
To protect piglets from PEDV, Sow transfers immunoglobulin through colostrum to their children until the piglets acquire adaptive immunity. Many reports suggest that IgA is important for protection of PEDV. AffiGEN PED IgA Ab ELISA measures preventive anti PED-virus IgA titers of sow and predict defense capability of piglets induced by passive antibody transfer.
Indications:
- Quantitative detection of PED IgA antibody
- Screening for defensive capacity against PEDV
Special Features:
- Easy sample collection
- Optimal screening method for defensive capacity of PEDV
- Specimen : Colostrum
- Reading Time : 1 hour and 45 minutes
- Survival rate of IgA positive confirmed group after challenge : see tables below
Quick Procedure:
- Prepare PED antigen coated test plate
- Dispense 100 μl of sample diluent into each well
- Dispense 10 μl of positive, negative control and samples to each well
- Incubate the plate at 37±1℃ for 60 minutes
- Wash the plate 5 times
- Dispense 100 μl of diluted enzyme conjugate to each well
- Incubate the wells for 30 minutes at 37±1℃
- Wash the plate 5 times
- Dispense 100 μl of substrate to each well
- Incubate the wells for 15 minutes at room temperature (18~25℃)
- Dispense 100 μl of stopping solution
- Measure the optical density (OD) at 450nm with reference wavelength at 620nm
- Cut off value = [0.35+ mean OD NC]