AffiPLATE® Carboxylated, 96 well Solid plates (Black PS)
AffiPLATE® Carboxylated plates are high-performance, 96-well microplates made from black polystyrene (PS). Each well surface is functionalized with carboxyl groups (-COOH), enabling covalent binding of biomolecules. The black PS composition is optimized for fluorescence applications, minimizing background signal and enhancing sensitivity.
Key Applications:
Biomolecule Immobilization:
Protein Binding: The carboxyl groups on AffiPLATE® Carboxylated plates facilitate covalent coupling with primary amines of proteins through carbodiimide chemistry (e.g., EDC/NHS). This strong attachment is ideal for assays requiring stable protein immobilization, such as enzyme-linked immunosorbent assays (ELISA) and protein-protein interaction studies.
Peptide Coupling: Peptides with free amino termini can be covalently linked to the carboxylated surface, making these plates suitable for peptide mapping, antibody epitope mapping, and peptide-based screening assays.
Nucleic Acid Attachment: Oligonucleotides with 5' amine modifications can be covalently attached to the plate surface. This is useful for hybridization assays, microarray applications, and DNA-protein interaction studies.
High-Throughput Screening (HTS):
Drug Discovery: The 96-well format of AffiPLATE® allows for high-throughput screening of drug candidates. The robust covalent attachment of target molecules ensures consistent and reproducible assay conditions across multiple wells and plates.
Compound Library Screening: These plates are suitable for screening large libraries of small molecules, peptides, or antibodies, allowing for the identification of potential therapeutic candidates with high specificity and low background noise.
Fluorescence-Based Assays:
Fluorescence Resonance Energy Transfer (FRET): The black PS minimizes autofluorescence and cross-talk between wells, making these plates ideal for FRET assays to study molecular interactions and conformational changes.
Fluorescent Immunoassays: AffiPLATE® Carboxylated plates support the development of sensitive fluorescent immunoassays. The high binding efficiency and low background ensure precise quantification of analytes in complex biological samples.
Diagnostic Applications:
Pathogen Detection: The ability to immobilize specific capture antibodies or antigens makes these plates suitable for developing diagnostic assays for infectious diseases. The high sensitivity and specificity are crucial for detecting low-abundance pathogens.
Biomarker Analysis: AffiPLATE® Carboxylated plates are used in the detection and quantification of disease biomarkers. The stability of the covalent attachment ensures reliable assay performance over time.
Research and Development:
Biochemical Assays: These plates are used in various biochemical assays where covalent immobilization of enzymes, substrates, or inhibitors is required. The stability of attachment supports kinetic studies and mechanistic investigations.
Cell-Free Assays: AffiPLATE® Carboxylated plates are suitable for cell-free systems where proteins or nucleic acids need to be immobilized for interaction studies, including transcription and translation assays.
Technical Specifications:
Material: Black Polystyrene (PS)
Well Count: 96 wells
Surface Chemistry: Carboxyl groups (-COOH)
Well Volume: Typically 300 µL per well
Compatibility: Compatible with standard microplate readers and robotic handling systems
Storage Conditions: Store in a dry environment at room temperature, avoid exposure to direct sunlight
Handling and Usage:
Preparation: Activate the carboxyl groups using a carbodiimide reagent (e.g., EDC) before coupling the desired biomolecule.
Washing: Use appropriate buffers to wash the wells post-coupling to remove unbound molecules.
Blocking: Block remaining reactive sites with a suitable blocking agent (e.g., BSA) to reduce nonspecific binding.
By leveraging the carboxyl-functionalized surface of AffiPLATE® Carboxylated, 96-well Solid Plates, researchers can achieve high-specificity immobilization of biomolecules, enhancing the performance and reliability of various biochemical and diagnostic assays.