AffiGEN® TransfectTM siRNA In Vitro 1000 Buffer (5X)
Introducing our cutting-edge Advanced AffiGEN® TransfectTM siRNA In Vitro 1000 Buffer (5X), precisely formulated to achieve optimal transfection efficiency and maximize gene expression knockdown when paired with AffiGEN® TransfectTM siRNA In Vitro 1000 and AffiGEN® TransfectTM siRNA In Vitro 1000 Plus. This innovative buffer is your key to success when diluting siRNA/DNA and combining them with AffiGEN® TransfectTM siRNA In Vitro 1000 (Plus) to create the most efficient transfection complex.
Product Details:
Product Name: AffiGEN® TransfectTM siRNA In Vitro 1000 Buffer (5X)
Volume: Each vial contains 8.0 mL of 5X concentrated stock solution, enabling you to prepare 40 mL of working solution at a 1X concentration.
Sufficient for up to 1000 transfections in a standard 24-well plate.
Storage:
Store at room temperature (RT).
With proper storage, this product maintains stability for a minimum of 12 months, and potentially even longer.
siRNA In Vitro Transfection Reagents Specifications and Guide Application
| Product | AffiGEN® TransfectTM siRNA In Vitro 1000 | AffiGEN® TransfectTM siRNA In Vitro 2000 | AffiGEN® TransfectTM siRNA In Vitro 3000 | AffiGEN® TransfectTM siRNA In Vitro 4000 | AffiGEN® TransfectTM siRNA In Vitro 1000 Plus |
| Nucleic Acid | siRNA | siRNA, miRNA, DNA | siRNA | siRNA | siRNA |
| Technology | Peptide-based | Non-liposomal | Liposome formulation | Polymer-based | Peptide-based |
| Efficiency | High | High | High | High | High |
| Cell compatibility | Wide | Wide | Wide | Wide | Wide |
| Off-target effects | Low | Low | Low | Low | Low |
| Cytotoxicity | Moderate | Moderate | Low | Very Low | Moderate |
| Package sizes | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL |
| Storage | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT |
| Applications | Gene silencing, RNAi | Gene silencing, miRNA delivery, DNA delivery | Gene silencing | Gene silencing | Gene silencing |
| Target Organisms | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells |
| Cell Types Suitable For | Wide range of cell types | Wide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells | Wide range of cell types | Wide range of cell types, including suspension cells and difficult-to-transfect cells | Wide range of cell types, including hard-to-transfect cells |
| Additional Notes | N/A | Can be used for in vivo applications | N/A | Optimized for siRNA delivery, but can also be used for DNA transfection | Effective at low concentrations |