Overview
TOP10 Chemically Competent Cell from AffiGEN is the competent cells obtained by the special process of Escherichia coli strain TOP10 with Streptomycin (StrR) resistance. It can be used for the chemical transformation of DNA with a conversion efficiency of up to 108 cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification, and can ensure stable inheritance of high-copy plasmids. At the same time, it can be used to construct clones and blue-white colony screening. TOP10 competent cell genotypes: F-mcrA △ (mrr-hsdRMS-mcrBC) φ80lacZ△M15 △lacX74 recA1 ara△139 △ (ara-leu) 7697 galU galK rpsL (StrR ) endA1 nupG.
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