AffiAB® Anti-dsRNA Monoclonal Antibody [J5] (Mouse IgG2b, κ)

AffiAB® Anti-dsRNA Monoclonal Antibody [J5] (Mouse IgG2b, κ) 

General Use:
For general laboratory use.

Shipping & Storage:

• Shipping: Shipped on gel packs
• Storage Conditions:

1. Upon reconstitution, store at –20°C to –80°C for long-term storage
2. Avoid freeze/thaw cycles; store in aliquots
3. With the addition of 10 mM sodium azide, the undiluted antibody (1 µg/µL) can be stored at 4°C for short-term use

• Shelf Life: 12 months from the date of delivery

Formulation & Reconstitution:

• Form: Lyophilized from a 1 mg/mL PBS solution
• Solubility:
  To prepare a 1 µg/µL antibody solution:

1. Add 200 µL of sterile, DNAse/RNAse-free distilled water to AFG-JBS-0003 200 µg.
2. Add 500 µL to AFG-JBS-0003 500 µg.

Note: Due to the lyophilization process, small amounts of denatured protein may be present as aggregates. These may affect certain applications, such as immunohistochemistry, by increasing background signal.
Recommendation: Spin down the reconstituted solution using a microcentrifuge and use the supernatant only for experiments.

Description:
The AffiAB® Anti-dsRNA Monoclonal Antibody J5 exhibits specificity and affinity similar to the well-established J2 clone (Schönborn et al., 1991), but with a different isotype (IgG2b). This feature provides greater flexibility for simultaneous labeling in multiplex assays, such as immunofluorescence microscopy.
J5 has been successfully used for the detection of dsRNA replicative intermediates of Infectious Pancreatic Necrosis Virus (IPNV) (Levican-Asenjo et al., 2019) and ECMV in Vero cells.

Applications:

• ELISA
• dsRNA Immunoblotting
• Immunofluorescence Microscopy

Important: For dsRNA immunoblotting, use polyacrylamide gel electrophoresis for nucleic acid separation. Detection sensitivity is significantly reduced when blotting from agarose gels.

Specificity:

• Recognizes double-stranded RNA (dsRNA) helices ≥40 base pairs
• Sequence- and nucleotide composition-independent recognition
• Binds all tested naturally occurring dsRNAs (40–50 species)
• Detects synthetic analogs: poly(I):poly(C) and poly(A):poly(U)

Affinity for poly(I):poly(C) is ~10-fold lower than for other dsRNA molecules

Antibody Characteristics:

• Species Origin: Mouse
• Heavy Chain Isotype: IgG2b
• Light Chain Isotype: Kappa

Quality Control:

• Activity: Validated by AN-ELISA