AffiVET® Avian Influenza Virus AIV H5 Antigen Rapid Tests
Species: Pourtry
Disease or Pathogen: Bird flu/Avian Influenza
Target: Antigen
Test Type: Rapid Tests
What is AIV H5 ?
Avian influenza A viruses can be classified as low and high pathogenic form based on the severity of the illness they cause. Avian influenza A subtype H5 viruses can be considered as “high pathogenic” strains on the basis of genetic features of the virus and the severity of the illness.
AffiVET® Avian Influenza type A antigen subtyping rapid test
AffiVET® Avian Influenza Virus Antigen 3line Test is a chromatographic immunoassay for the qualitative detection of avian influenza type A virus antigen and a specific subtype antigen in avian cloaca, trachea, kidney or feces. Avian influenza A viruses can be classified as low and high pathogenic form based on the severity of the illness they cause. Avian influenza A subtype H5 viruses can be considered as “high pathogenic” strains on the basis of genetic features of the virus and the severity of the illness. Both test lines on the device mean the sample positive of AIV type A and its specific subtype H5. Only AIV Ag line on the device means sample positive of AIV type A.
Indications
• Field monitoring of Avian Influenza virus and its subtypes H5
• Tentative diagnosis for swift control in suspected outbreak situation
• Differentiation between low pathogenic and high pathogenic
Special Features
• Discriminate between a common AIV and a specific subtype AIV
• Differential diagnosis of AIV subtype H5
• World’s first commercialized rapid test kit for AIV H5
• High sensitivity and high specificity
• Applicable to various species
Material required
Storage & Stability
Precautions
Sample Collection & Preparation
• The samples should be tested immediately after collection.
• If samples are not tested immediately, they should be stored at 2~8°C for 24 hours. For longer storage, freeze at -20°C or below.
• The amount of fecal swab may affect the results. It is required to follow the swab amount of feces as shown in the picture. Excessive fecal amount may induce a false positive result and slow migration.
Test Procedure
Result Interpretation
Negative result
Only control (“C”) line appears in the result window.
Positive result
AIV type A and AIV subtype H5 Ag Positive
The presence of Control line “C”, Test line “1” and "2" within the result window indicates an avian influenza virus subtype H5 infection.
AIV type A Ag Positive & AIV subtype H5 Ag non-determined
The presence of Control line “C” and Test line “2” within the result window indicates an avian influenza virus infection. However, it is not determined if subtype H5 infected or not. It is recommended that the sample should be tested using other more sensitive diagnostic test (e.g. Real-time PCR) for confirmation.
Invalid Result
If the Control line “C” does not appear, the result might be considered invalid. The sample should be retested.
Test Limitations
• The reading window may show a light pink background coloration; this will not affect the accuracy of the results.
• AffiGEN Inc. and its distributors cannot be held responsible for the consequences of misuse or misinterpretation of the results given by the test.
Cross Reactivity
AIV subtype H5 Ag test (Test line “1”) showed no cross-reactivity with other H subtype (H1 ~ H4, H6 ~ H15) antigens of avian influenza virus type A, Newcastle Disease virus, Infectious Bronchitis virus and other microorganisms.
Colonne1 | H5 Ag |
A/PR/8/34(H1N1) | - |
A/Singapore/1/57(H2N2) | - |
A/Duck/Ukrine/1/63(H3N8) | - |
A/Duck/Czechoslovakia/56(H4N6) | POS |
A/Duck/Hongkong/820/80(H5N3) | - |
A/Shearwater/Australia/1/72(H6N5) | - |
A/Duck/Hong Kong/301/78(H7N1) | - |
A/Turkey/Ontario/6118/68(H8N4) | - |
A/Turkey/Wisconsin/1/66(H9N2) | - |
A/Chicken/Germany/N/49(H10N7) | - |
A/Duck/England/56(H11N6) | - |
A/Duck/Alberta/60/76(H12N5) | - |
A/Gull/Maryland/704/77(H13N6) | - |
A/Mallard/Gurjev/263/82(H14N5) | - |
A/Shearwater/West Australia/2576/ 79(H15N9) | - |
Ordering Information
Cat. No. | Description | Type | Packing size |
AFG-VR-73-10 | Rapid H5 AIV Antigen | Multi device | 10 Tests |
AFG-VR-73-30 | Rapid H5 AIV Antigen | Multi device | 30 Tests |