AffiPURE® Plasmid Mini Kit

AffiPURE®  Plasmid Mini Kit

Take 1 - 5 ml of overnight bacteria culture and lyse the cells by an optimized SDS-alkaline method. With this kit, the extraction of multiple samples can be completed within 30 min without involving phenol-chloroform extraction or ethanol precipitation. The kit uses a unique spin column to specifically bind plasmid DNA in the solution in a highly efficient manner under high-salt and low-pH conditions and remove proteins, genomic DNA, RNA, and other impurities to the greatest extent. In the end, pure plasmid DNA is eluted from the silica membrane with a low-salt, high-pH eluent. Each column adsorbs up to 35 µg of plasmid DNA. The purified plasmid DNA is ready for use in biological experiments such as enzyme digestion, PCR, sequencing, ligation, transformation, transfection, and common passaging cells experiments.

Components:

Components	AFG-VZ-DC201-01 100 Reactions
RNase A	150 μL
Buffer P1	30 mL
Buffer P2	30 mL
Buffer P3	40 mL
Buffer PW1	60 mL
Buffer PW2	2 x 20 mL
Elution Buffer	20 mL
AffiPURE DNA Mini Columns	100
Collection Tubes 2 mL	100

 

Note:

RNase A: Degrade RNA in the sample.
Buffer P1: Suspend bacteria. Add RNase A to Buffer P1 before the first use.
Buffer P2: Lyse bacteria (containing SDS/NaOH).
Buffer P3: Neutralizing buffer.
Buffer PW1: Remove proteins and other impurities from plasmids.
Buffer PW2: Remove residual salt ions from plasmids.
Elution Buffer: Elute DNA.
AffiPURE DNA Mini Columns: Adsorb plasmid DNA.
Collection Tubes 2 mL: Collect filtrate.

 

Storage:

Store at 15 ~ 25°C and transport at room temperature.
If ambient temperatures often exceed 25°C, we suggest storing RNase A at 2 ~ 8°C.
After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2 ~ 8°C.
 

Applications:

This product is is suitable for 1 - 5 ml of overnight bacterial cultures.
When preparing low-copy plasmids, scale up the volumes of Buffer P1, Buffer P2, and Buffer P3, and increase the volume of bacterial solution to 5 - 10 ml.