AffiPCR® One Step RT-PCR Kit (Dye Plus) V2
AffiPCR® One Step RT-PCR Kit (Dye Plus) V2 is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains HiScript II Reverse Transcriptase, RNase inhibitor, and Champagne Taq plus hot-start DNA Polymerase, which enables high-sensitive total RNA detection (as little as 1 pg) and long-fragment amplification (as long as 10 kb). The 2X One Step Mix (Dye Plus) contains an opimized buffer, dNTPs, and Loading Dye.
Content of the kit:
| Components |
AFG-VZ-P612-01 50 rxn (50 μl/rxn) |
| RNase free ddH2O | 1 ml × 2 |
| 2X One Step Mix (Dye Plus) a | 625 μl × 2 |
| One Step Enzyme Mix b | 125 µl |
a. Contains dNTPs and Loading Dye.
b. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.
Storage:
All components should be stored at -20°C.