AffiGEN® TransfectTM siRNA In Vitro 4000 Buffer (5X)
Our Enhanced AffiGEN® TransfectTM siRNA In Vitro 4000 Buffer (5X) has been meticulously formulated to achieve the highest level of transfection efficiency and maximal gene expression knockdown, specifically optimized for use with our AffiGEN® TransfectTM siRNA In Vitro 4000. This advanced buffer is the ideal solution for diluting siRNA/DNA and the AffiGEN® TransfectTM siRNA In Vitro 4000, ensuring the formation of the most efficient transfection complex.
Product Details:
Product Name: AffiGEN® TransfectTM siRNA In Vitro 4000 Buffer (5X)
Volume: 8.0 mL per vial, yielding a 40 mL working solution at 1X concentration.
Designed for up to 1000 transfections in a standard 24-well plate.
Storage:
Store at room temperature (RT).
Under proper storage conditions, this product maintains stability for a minimum of 12 months and potentially even longer.
siRNA In Vitro Transfection Reagents Specifications and Guide Application
| Product | AffiGEN® TransfectTM siRNA In Vitro 1000 | AffiGEN® TransfectTM siRNA In Vitro 2000 | AffiGEN® TransfectTM siRNA In Vitro 3000 | AffiGEN® TransfectTM siRNA In Vitro 4000 | AffiGEN® TransfectTM siRNA In Vitro 1000 Plus |
| Nucleic Acid | siRNA | siRNA, miRNA, DNA | siRNA | siRNA | siRNA |
| Technology | Peptide-based | Non-liposomal | Liposome formulation | Polymer-based | Peptide-based |
| Efficiency | High | High | High | High | High |
| Cell compatibility | Wide | Wide | Wide | Wide | Wide |
| Off-target effects | Low | Low | Low | Low | Low |
| Cytotoxicity | Moderate | Moderate | Low | Very Low | Moderate |
| Package sizes | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL |
| Storage | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT |
| Applications | Gene silencing, RNAi | Gene silencing, miRNA delivery, DNA delivery | Gene silencing | Gene silencing | Gene silencing |
| Target Organisms | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells |
| Cell Types Suitable For | Wide range of cell types | Wide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells | Wide range of cell types | Wide range of cell types, including suspension cells and difficult-to-transfect cells | Wide range of cell types, including hard-to-transfect cells |
| Additional Notes | N/A | Can be used for in vivo applications | N/A | Optimized for siRNA delivery, but can also be used for DNA transfection | Effective at low concentrations |