AffiGEN® TransfectTM siRNA In Vitro 4000
Revolutionary Hybrid Formulation for Optimal siRNA Delivery
The AffiGEN® TransfectTM siRNA In Vitro 4000 stands at the forefront of genetic research tools, thanks to its innovative hybrid composition that combines the best aspects of liposome and polymer technologies. This cutting-edge approach ensures not only superior siRNA delivery efficiency but also minimal to no cytotoxicity, setting a new standard in the field.
Contents and Specifications:
AffiGEN® TransfectTM siRNA In Vitro 4000: 1.0 mL, designed for approximately 1330 reactions, assuming the use of 5.0 pmol siRNA per reaction in a 24-well plate format.
AffiGEN® TransfectTM siRNA In Vitro 4000 Buffer (5X): 8.0 mL, engineered to enhance transfection efficiency, yields 40 mL of ready-to-use solution.
Storage Guidelines:
AffiGEN® TransfectTM siRNA In Vitro 4000: Refrigerate at 4 °C.
AffiGEN® TransfectTM siRNA In Vitro 4000 Buffer (5X): Store at room temperature (RT).
Adhering to these conditions will ensure the product remains stable and effective for 12 months or more.
Distinguished Advantages:
Leveraging proprietary technologies in lipid-conjugation and polymer synthesis, AffiGEN® TransfectTM siRNA In Vitro 4000 distinguishes itself from competitors with several key benefits:
- Unmatched Efficiency: Ideal for transfecting cells that are traditionally challenging, ensuring high-level performance across a diverse range of mammalian cells, all while maintaining virtually undetectable cytotoxicity levels.
- Cost-Effectiveness: Offers a superior, budget-friendly alternative without compromising on quality or efficacy.
Additional Benefits:
- Free from sera interference, facilitating consistent and reliable results.
- Demonstrates exceptional stability for long-term storage and use.
- Simplified and expedited protocol, saving valuable time in the lab.
- Fully compatible with high-throughput screening methods, making it perfect for extensive research projects.
AffiGEN® TransfectTM siRNA In Vitro 4000 merges unparalleled efficiency with ease of use, making it an indispensable tool for researchers aiming to push the boundaries of genetic discovery.
siRNA In Vitro Transfection Reagents Specifications and Guide Application
| Product | AffiGEN® TransfectTM siRNA In Vitro 1000 | AffiGEN® TransfectTM siRNA In Vitro 2000 | AffiGEN® TransfectTM siRNA In Vitro 3000 | AffiGEN® TransfectTM siRNA In Vitro 4000 | AffiGEN® TransfectTM siRNA In Vitro 1000 Plus |
| Nucleic Acid | siRNA | siRNA, miRNA, DNA | siRNA | siRNA | siRNA |
| Technology | Peptide-based | Non-liposomal | Liposome formulation | Polymer-based | Peptide-based |
| Efficiency | High | High | High | High | High |
| Cell compatibility | Wide | Wide | Wide | Wide | Wide |
| Off-target effects | Low | Low | Low | Low | Low |
| Cytotoxicity | Moderate | Moderate | Low | Very Low | Moderate |
| Package sizes | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL |
| Storage | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT |
| Applications | Gene silencing, RNAi | Gene silencing, miRNA delivery, DNA delivery | Gene silencing | Gene silencing | Gene silencing |
| Target Organisms | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells |
| Cell Types Suitable For | Wide range of cell types | Wide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells | Wide range of cell types | Wide range of cell types, including suspension cells and difficult-to-transfect cells | Wide range of cell types, including hard-to-transfect cells |
| Additional Notes | N/A | Can be used for in vivo applications | N/A | Optimized for siRNA delivery, but can also be used for DNA transfection | Effective at low concentrations |