AffiGEN® TransfectTM siRNA In Vitro 3000 Buffer (5X)
Introducing our cutting-edge Advanced AffiGEN® TransfectTM siRNA In Vitro 3000 Buffer (5X), thoughtfully crafted to elevate transfection efficiency to its pinnacle and achieve unparalleled gene expression knockdown. This extraordinary buffer is exclusively designed to complement our AffiGEN® TransfectTM siRNA In Vitro 3000, ensuring optimal results when diluting siRNA/DNA and combining them with AffiGEN® TransfectTM siRNA In Vitro 3000 to form the most effective transfection complex.
Product Details:
Product Name: AffiGEN® TransfectTM siRNA In Vitro 3000 Buffer (5X)
Volume: Each vial contains 8.0 mL of 5X concentrated stock solution, yielding 40 mL of working solution at 1X concentration.
Capable of facilitating up to 1,200 transfections in a standard 24-well plate.
Storage:
Store at room temperature (RT).
When stored under proper conditions, this product maintains stability for a minimum of 12 months, and potentially even longer.
siRNA In Vitro Transfection Reagents Specifications and Guide Application
| Product | AffiGEN® TransfectTM siRNA In Vitro 1000 | AffiGEN® TransfectTM siRNA In Vitro 2000 | AffiGEN® TransfectTM siRNA In Vitro 3000 | AffiGEN® TransfectTM siRNA In Vitro 4000 | AffiGEN® TransfectTM siRNA In Vitro 1000 Plus |
| Nucleic Acid | siRNA | siRNA, miRNA, DNA | siRNA | siRNA | siRNA |
| Technology | Peptide-based | Non-liposomal | Liposome formulation | Polymer-based | Peptide-based |
| Efficiency | High | High | High | High | High |
| Cell compatibility | Wide | Wide | Wide | Wide | Wide |
| Off-target effects | Low | Low | Low | Low | Low |
| Cytotoxicity | Moderate | Moderate | Low | Very Low | Moderate |
| Package sizes | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL |
| Storage | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT |
| Applications | Gene silencing, RNAi | Gene silencing, miRNA delivery, DNA delivery | Gene silencing | Gene silencing | Gene silencing |
| Target Organisms | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells |
| Cell Types Suitable For | Wide range of cell types | Wide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells | Wide range of cell types | Wide range of cell types, including suspension cells and difficult-to-transfect cells | Wide range of cell types, including hard-to-transfect cells |
| Additional Notes | N/A | Can be used for in vivo applications | N/A | Optimized for siRNA delivery, but can also be used for DNA transfection | Effective at low concentrations |