Chargez l'image dans la visionneuse de la galerie, AffiGEN® Transfect(TM) DNA In Vitro 3000
Chargez l'image dans la visionneuse de la galerie, AffiGEN® Transfect(TM) DNA In Vitro 3000
Chargez l'image dans la visionneuse de la galerie, AffiGEN® Transfect(TM) DNA In Vitro 3000

SKU: AFG-SNG-TR-009

AffiGEN® Transfect(TM) DNA In Vitro 3000

Vendeuse AffiGEN
Prix ​​habituel €197,00 EUR
Prix ​​de vente €197,00 EUR Prix ​​habituel
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AffiGEN® TransfectTM DNA In Vitro 3000

Product Description

AffiGEN® TransfectTM DNA In Vitro 3000 harnesses our innovative and proprietary lipid conjugation technology. Specifically tailored and formulated with the inclusion of exclusive enhancers for transfecting HEK293 cells and other mammalian cells (as depicted in Figure 1), this 2nd generation liposome-based DNA transfection reagent represents a significant advancement. AffiGEN® TransfectTM DNA In Vitro 3000 achieves exceptionally high transfection efficiencies for HEK293-related cells and numerous mammalian cell types, all while minimizing cytotoxicity. Upgraded from its previous version with refined chemistry, AffiGEN® TransfectTM DNA In Vitro 3000 excels with a 3~4 times increase in gene delivery efficiency. Each unit of AffiGEN® TransfectTM DNA In Vitro 3000 reagent, with a volume of 1.0 ml, is suitable for more than 600 transfections in 24-well plates or more than 300 transfections in 6-well plates.

Features

 - The preferred choice for challenging-to-transfect cells.
 - Achieves exceptional virus production yields.
 - Equally effective for very long DNA molecules (up to 180 kb).
 - Suitable for suspension 293 cells (e.g., 293F, 293H, etc.).
 - Achieves high levels of recombinant protein production.
 - Efficiency is enhanced in the presence of serum and antibiotics for 293 cells.
 - Exceptional efficiency for both single DNA transfection and multi-DNA co-transfection.
 - A highly cost-effective solution.

Storage Condition

Store AffiGEN® TransfectTM DNA In Vitro 3000 at 4°C. When stored correctly, the product remains stable for 12 months or longer.


DNA In Vitro Transfection Reagents Specifications

Product Nucleic Acid Type Delivery Method Toxicity Efficiency Serum Tolerance
AffiGEN® Transfect™ 1000 - Mammalian Cell DNA Calcium phosphate Precipitation High High High
AffiGEN® Transfect™ DNA In Vitro 2000 V1 DNA, siRNA Polymer Polymer complex Low High Low
AffiGEN® Transfect™ DNA In Vitro 2000 V2 DNA, siRNA Polymer Polymer complex Moderate Higher Medium
AffiGEN® Transfect™ DNA In Vitro 2000 Plus DNA Polymer Polymer complex Low Higher Medium
AffiGEN® Transfect™ DNA In Vitro 3000 DNA Liposomal Liposome-mediated Moderate High High
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit DNA, siRNA Liposomal Liposome-mediated Very low High High
AffiGEN® Transfect™ DNA In Vitro 5000 DNA, siRNA Polymer Polymer complex Moderate High High

 

DNA In Vitro Transfection Reagents Application Guide

Product Applications Cell Types Cell lines Suitable for Additional Notes
AffiGEN® Transfect™ 1000 - Mammalian Cell Protein production, stable cell line generation, viral vector production Adherent, some suspension HEK293, HepG2, CHO Large-scale transfection, cost-effective
Lower calcium concentration than traditional methods
AffiGEN® Transfect™ DNA In Vitro 2000 V1 Gene expression studies, protein production, virus production Most, including sensitive Various Sensitive cell lines, maximizing cell viability
Multiple formulations available
AffiGEN® Transfect™ DNA In Vitro 2000 V2 Gene expression studies, protein production, virus production Most, including sensitive Various Balancing efficiency and viability
Released from DNA complex
AffiGEN® Transfect™ DNA In Vitro 2000 Plus Protein production, viral vector production Most, including sensitive Various Long DNA, high efficiency
Proprietary polymer
AffiGEN® Transfect™ DNA In Vitro 3000 Viral vector production, antibody production, protein production Adherent, suspension Various Lentivirus production, high efficiency
Enhanced conjugate
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit Protein production, gene editing, virus production Most, including sensitive Various Low toxicity, high efficiency
Fluorinated cationic lipids
AffiGEN® Transfect™ DNA In Vitro 5000 Gene expression studies, protein production, virus production Most, including sensitive Various Common cell lines, biodegradable
Biodegradable polycationic polymer