AffiGEN® TransfectTM DNA In Vitro 2000 Plus
Product Description
AffiGEN® TransfectTM DNA In Vitro 2000 Plus represents an upgraded version of the GenJet™ DNA In Vitro Transfection Reagent. AffiGEN® TransfectTM DNA In Vitro 2000 Plus has been enhanced through the incorporation of a booster peptide, resulting in a remarkable up to 20 times higher transfection efficiency across a wide range of mammalian cells when compared to its previous iteration. AffiGEN® TransfectTM DNA In Vitro 2000 Plus has demonstrated its capability to efficiently deliver genes to various well-established cell lines, as well as primary cells, including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines such as Sf9 and Sf21, and various other eukaryotic cell lines, all with reduced toxicity. Each unit of AffiGEN® TransfectTM DNA In Vitro 2000 Plus reagent, with a volume of 1.0 ml, is sufficient for more than 600 transfections in 24-well plates or more than 300 transfections in 6-well plates.
Features
- Achieves cell-dependent 3~20 times higher efficiency.
- Remains the top choice for challenging-to-transfect cells.
- Equally effective for very long DNA molecules (up to 46 kb).
- Demonstrates increased efficiency even in the presence of serum and antibiotics for most cell types.
- Yields exceptionally high levels of recombinant protein production.
Storage Condition
Store AffiGEN® TransfectTM DNA In Vitro 2000 Plus at 4°C. When stored properly, the product maintains stability for 12 months or longer.
DNA In Vitro Transfection Reagents Specifications
| Product | Nucleic Acid | Type | Delivery Method | Toxicity | Efficiency | Serum Tolerance |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | DNA | Calcium phosphate | Precipitation | High | High | High |
| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | DNA, siRNA | Polymer | Polymer complex | Low | High | Low |
| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | DNA, siRNA | Polymer | Polymer complex | Moderate | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | DNA | Polymer | Polymer complex | Low | Higher | Medium |
| AffiGEN® Transfect™ DNA In Vitro 3000 | DNA | Liposomal | Liposome-mediated | Moderate | High | High |
| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | DNA, siRNA | Liposomal | Liposome-mediated | Very low | High | High |
| AffiGEN® Transfect™ DNA In Vitro 5000 | DNA, siRNA | Polymer | Polymer complex | Moderate | High | High |
DNA In Vitro Transfection Reagents Application Guide
| Product | Applications | Cell Types | Cell lines | Suitable for | Additional Notes |
| AffiGEN® Transfect™ 1000 - Mammalian Cell | Protein production, stable cell line generation, viral vector production | Adherent, some suspension | HEK293, HepG2, CHO | Large-scale transfection, cost-effective |
Lower calcium concentration than traditional methods
|
| AffiGEN® Transfect™ DNA In Vitro 2000 V1 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Sensitive cell lines, maximizing cell viability |
Multiple formulations available
|
| AffiGEN® Transfect™ DNA In Vitro 2000 V2 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Balancing efficiency and viability |
Released from DNA complex
|
| AffiGEN® Transfect™ DNA In Vitro 2000 Plus | Protein production, viral vector production | Most, including sensitive | Various | Long DNA, high efficiency |
Proprietary polymer
|
| AffiGEN® Transfect™ DNA In Vitro 3000 | Viral vector production, antibody production, protein production | Adherent, suspension | Various | Lentivirus production, high efficiency |
Enhanced conjugate
|
| AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | Protein production, gene editing, virus production | Most, including sensitive | Various | Low toxicity, high efficiency |
Fluorinated cationic lipids
|
| AffiGEN® Transfect™ DNA In Vitro 5000 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Common cell lines, biodegradable |
Biodegradable polycationic polymer
|