SKU: AFG-VZ-N408-01

AffiNGS® Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat)

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Prix ​​habituel €1.123,00 EUR
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AffiNGS® Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat)

AffiNGS® Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat) is a kit for removing Globin mRNA and rRNA from  total RNA in blood samples. This kit is compatible with 0.01 - 1 µg blood total RNA. The total RNA sample undergoes Globin mRNA and rRNA hybridize with probe, RNase H digestion, DNase I digestion, etc. Finally, Globin mRNA and rRNA (including cytoplasmic rRNA and mitochondrial rRNA) are removed from total RNA, leaving other mRNA and non-coding RNA, which can be used for the analysis of non-coding RNA such an LncRNA. This kit is compatible with partially degraded RNA samples, and the obtained products are suitable for RNA library construction and the other experiments.

 Components:

Components AFG-VZ-N408-01 (24 rxns) AFG-VZ-N408-02 (96rxns)
Globin and rRNAA Probe (H/M/R) 24 µl 96µl
Probe Buffer 72 µl 288 µl
Rnase H Buffer 2 72 µl 288 µl
Rnase H Mix 48 µl 192 µl
Dnase I Buffer 696 µl 4 x 696 µl
Dnase I 24 µl 96 µl

Nuclease-free ddH2O

1 ml 4 x 1 ml

 

Storage:

Store at -30 ~ -15° and transport at ≤0°C.