AffiVET® Poultry AIV Antibody ELISA Kit

AffiVET® Poultry AIV Antibody ELISA Kit

Species: Poultry

Disease or Pathogen: Bird flu/Avian Influenza

Target: Antibody

Test Type: Elisa Kit

Avian Influenza type A virus antibody:

Wild birds and some poultry infected by HPAI show no clinical signs, and their virus shedding amount is too small to be detected by an antigen capture immunoassay. The AffiGen AIV Ab ELISA has been developed for screening these latent AIV carriers by detecting AIV antibodies.

Indications:

• Avian influenza virus antibody detection
• Monitoring of Avian Influenza virus carrier
• Antibody detection for migratory bird surveillance

Special Features:

• No cross-reaction with other avian virus positive sera
• Applicable to various species
• Specimen: Serum, plasma or egg yolk
• No sample dilution is required
• Reading time: 45 minutes
• Sensitivity: Chicken 98.2%, Duck 97.5%, Turkey 97.1%
• Specificity: Chicken 97.3%, Duck 93.8%, Turkey 100%

Quick Procedure:

1. Prepare AIV NP antigen coated test plate
2. Add 50 μl of controls and sample to wells
3. Add 50 μl of anti AIV antibody-HRP to each well
4. Incubate plate for 30 minutes at 37℃
5. Wash plate 6 times
6. Add 100 μl of substrate (Ready to use) and incubate the wells for 10 minutes at room temperature
7. Add 100 μl of stopping solution
8. Measure the optical density (OD) at 450nm with reference wavelength at 620 nm
9. PI value=[1-(OD sample/mean OD NC)] x 100