AffiPURE® Plant Total RNA Isolation Kit (Polysaccharides / Polyphenolics-Rich)
This kit is suitable for the rapid extraction of total RNA from plant tissues, especially those rich in polysaccharides, polyphenols, or starch (e.g., cotton, mature rice, pine needles,populus, loquat, potato, banana, grape, apple, pear, China rose, buckwheat seeds, and Arabidopsis thaliana seeds), with extraction protocols provided for common plant tissues (e.g., Arabidopsis thaliana and tobacco). The kit is based on silica gel membrane purification technology without time-consuming alcohol precipitation and does not require phenol/chloroform, or any other toxic reagent during the extraction process. AffiPURE® gDNA-Filter Columns II can effectively remove impurities, adsorbing and removing genomic DNA. AffiPURE® RNA Columns IV can efficiently bind RNA, and with an optimized buffer the obtained total RNA has high purity. The isolated RNA has no proteins or other impurities contamination. It can be used for RT-PCR, Real-Time PCR, Microarrarys, Northern Blot, Dot Blot, Poly(A) screening, in vitro translation, RNase protection assay, molecular cloning and other downstream experiments.
Components:
| Components |
AFG-VZ-RC401-01 50 Reactions |
| Buffer PRL | 30 mL |
| Buffer PRLPlus | 25 mL |
| Buffer PRW1 | 40 mL |
| Buffer PRW2 | 12 mL |
| RNase-free ddH2O | 10 mL |
| AffiPURE gDNA-Filter Columns II (each in a 2 ml Collection Tube) | 50 |
| AffiPURE RNA Columns Ⅳ (each in a 2 ml Collection Tube) | 50 |
| Collection Tubes 2 ml | 50 |
| RNase-free Collection Tubes 1.5 ml | 50 |
Note:
Buffer PRL: Provide the environment required for plant tissues rich in polysaccharides and polyphenols.Buffer PRLPlus: Provide the environment required for the separation of RNA and DNA (polysaccharide- and polyphenol-rich plants).
Provide the environment required for the lysis of plant tissues and separation of RNA and DNA (common plants).
Buffer PRW1: Remove proteins and DNA impurities. Buffer PRW2: Remove residual salt ions.
RNase-free ddH2O: Elute total RNA.
AffiPURE gDNA-Filter Columns II: Adsorb DNA and RNA, and remove lysis impurities.
AffiPURE RNA Columns IV: Specifically adsorb RNA.
Collection Tubes 2 mL: Collect filtrate.
RNase-free Collection Tubes 1.5 mL: Collect RNA.
Storage:
Store at 15 ~ 25°C and transport at room temperature.
Applications:
50 - 100 mg of plant samples, such as pine needles and wheat leaves.10 - 20 mg of seed samples, such as red bean seeds and wheat seeds.