AffiPCR® Select RT SuperMix for qPCR (+gDNA wiper) V2
AffiPCR® HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. The HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) is specially designed for two-step qRT-PCR. The 4X gDNA wiper Mix in the product can rapidly remove the residual genomic DNA contamination and ensure more reliable subsequent quantitative results. 5X HiScript II Select qRT SuperMix II contains Buffer, dNTP mix, HiScript II Reverse Transcriptase and RNase inhibitor. Oligo (dT)23VN, Random primers or Gene Specific Primers (GSP) can be selected as reverse transcription primers according to requirements. Oligo (dT)23VN has stronger anchoring ability to Poly A+ mRNA than Oligo(dT)18, making reverse transcription more efficient. 5X HiScript II Select qRT SuperMix does not freeze at -20°C, and the reaction proceeds quickly after adding template RNA and primers. The RT product is compatible with dye-based and probe-based qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for high-performance gene expression analysis.