SKU: AFG-VZ-P504-d1

AffiPCR® EVO HS Super-Fidelity DNA Polymerase

Vendeuse AffiGEN
Prix ​​habituel $142.00 CAD
Prix ​​de vente $142.00 CAD Prix ​​habituel
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AffiPCR® EVO HS Super-Fidelity DNA Polymerase

AffiPCR® EVO HS Super-Fidelity DNA Polymerase adds a unique elongation factor and deeply optimizes the reaction system, which further improves its amplification stability, fidelity and long-fragment amplification ability. Simple templates such as λDNA and plasmids can effectively amplify long fragments. Up to 40 kb; complex templates such as genomic DNA efficiently amplify fragments up to 20 kb. Two monoclonal antibodies that can inhibit its 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature are added to Phanta EVO HS, which can perform highly specific Hot Start PCR . The enzyme has 5'→3' polymerase activity and 3'→5' exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress and topological cloning kits (AffiGEN#AFG-VZ-C112/AFG-VZ-C113/AFG-VZ-C115/AFG-VZ-C601).

Components:

Components AFG-VZ-P504-d1 AFG-VZ-P504-d3
AffiPCR® EVO HS Super-Fidelity DNA Polymerase (1 U/μl) 100 μl 10 x 100 μl
5 × EVO Buffer (with 10 mM MgCl2) 1.25 ml 10 x 1.25 ml
25 mM MgCl2 1 ml 10 x 1 ml
dNTP Mix (10 mM each) 100 µl 10 x 100 µl
5 × PCR Enhancer 500 µl 10 x 500 µl
10 × Loading buffer 1.25 ml 10 x 1.25 ml

 

Equivalence:

AFG-VZ-P504 AffiGEN AffiPCR® EVO HS Super-Fidelity DNA Polymerase
F549S/F549L/F549XL Thermo Fisher Phusion Hot Start I DNA Polymerase