AffiPCR® Animal Detection Probe Master Mix
AffiPCR® Animal Detection Probe Master Mix is a specialized master mix for probe qPCR to detect African swine fever virus (ASFV). Only need to add extra primers, probes and templates. This master mix utilizes an upgraded hot start Taq DNA polymerase with a carefully optimized buffer to increase detection sensitivity for low-concentration templates. This product can eliminate contamination in the reaction system to ensure the accuracy of the results. It is also compatible with fast program, reducing test time.
Components:
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Components
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AFG-VZ-QV110-EN01
(400 rxns/25 μl reaction)
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AFG-VZ-QV110-EN02
(800 rxns/25 μl reaction)
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2 × Animal Detection Probe Master Mixa
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5 × 1 ml
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10 ml
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50 × ROX Reference Dye 1b
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200 μl
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400 μl
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50 × ROX Reference Dye 2b
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200 μl
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400 μl
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a. It includes dNTP Mix, Mg²+ , hot start Taq DNA polymerase, etc..
b. It is used to correct the error of fluorescence signals between wells. Use 50 × ROX Reference Dye 1 for ABI 7900HT/7300 Real-Time PCR System and StepOnePlus; Use 50 × ROX Reference Dye 2 for ABI 7500, 7500 Fast Real-Time PCR System, and Stratagene Mx3000P. Don't use ROX for Roche and Bio-Rad Real-Time PCR instruments.
Storage:
Store at -30°C ~ -15°C and transport at ≤0°C.
Applications:
It is suitable for probe-based qPCR detection of DNA template.