AffiPCR® 2X Flash Master Mix

AffiPCR® 2X Flash Master Mix

The AffiPCR® 2X Flash Master Mix is a convenient and reliable PCR solution. It combines two high-quality reagents for DNA amplification with two buffers and two Mg²⁺ concentrations, giving you the superior results you expect without the need to prepare separate solutions. Accurate and reliable amplification every time.

The AffiPCR® 2X Flash Master Mix is a new generation superior enzyme. Through directed optimization of Flash Master Mix, The AffiPCR® Flash Master Mix DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec/kb) while maintaining high fidelity and yield. The amplification error rate of AffiPCR® Flash Super-Fidelity DNA Polymerase is 81-fold lower than that of conventional Taq DNA Polymerase. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling it to perform hot start PCR with great specificity. It contains all required reaction components (AffiPCR® Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. Amplification will generate blunt-ended products, which are compatible with ClonExpress kits and TOPO cloning kit.

Storage Conditions:

Store at temperatures between -30°C and -15°C, and ensure transportation at ≤0°C. After thawing, they can be stored at 4°C for up to three months, maintaining their quality and efficacy.

Equivalence: