AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X)
Product Description
AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X) has been specifically designed to complement the AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit (Cat# AFG-SNG-TR-11 & AFG-SNG-TR-12), ensuring maximal DNA transfection efficiency and optimal gene silencing outcomes. Prior to use, it is necessary to dilute the AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X) by adding 4 parts of ddH2O. The resulting AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer working solution is employed to dilute DNA, siRNA, and the AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Reagent to form the most effective transfection complex.
Package
Each vial of AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X) contains 8.0 mL of the solution, which, when appropriately diluted, yields a 40 mL working solution (1X). This quantity is sufficient for approximately 600 transfections in a 24-well plate.
Storage
Store AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5X) at room temperature (RT). When stored under proper conditions, the product remains stable for 12 months or longer.
DNA In Vitro Transfection Reagents Specifications
| Product | Type | Toxicity | Efficiency | Serum Tolerance | Delivery Method | Transfection Type | Package | Shelf Life |
| AffiGEN® TransfectTM 1000 - Mammalian Cell | DNA | High | High | Low | Calcium Phosphate Precipitation | Non-liposomal | 1 mL, 5X 1 mL | 2 years at 4°C |
| AffiGEN® TransfectTM DNA In Vitro 2000 V1 | DNA | Low | High | High | Liposomal | Cationic Liposomal | 1 mL, 5X 1 mL | 2 years at 4°C |
| AffiGEN® TransfectTM DNA In Vitro 2000 V2 | DNA | Moderate | Moderate | Moderate | Liposomal | Cationic Liposomal | 1 mL, 5X 1 mL | 2 years at 4°C |
| AffiGEN® TransfectTM DNA In Vitro 2000 Plus | DNA | Moderate | High | Moderate | Liposomal | Cationic Liposomal | 1 mL, 5X 1 mL | 2 years at 4°C |
| AffiGEN® TransfectTM DNA In Vitro 3000 | DNA | Low | High | High | Liposomal | Cationic Liposomal | 1 mL, 5X 1 mL | 2 years at 4°C |
| AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit | DNA & siRNA | Low | Moderate | High | Polymer-based | N/A | 1 mL, 5X 1 mL | 2 years at 4°C |
| AffiGEN® TransfectTM DNA In Vitro 5000 | DNA | Low | High | High | Liposomal | Cationic Liposomal | 1 mL, 5X 1 mL | 2 years at 4°C |
DNA In Vitro Transfection Reagents Application Guide
| Product | Target Organisms | Applications | Cell Types | Suitable for | Additional Notes |
| AffiGEN® TransfectTM 1000 - Mammalian Cell | Wide range, including hard-to-transfect mammalian cells | Gene expression, protein production | Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) | Virus, antibody and protein production | No serum required, simple protocol, can be cytotoxic in some cell lines |
| AffiGEN® TransfectTM DNA In Vitro 2000 V1 | Mammalian cells (HEK293, CHO, COS, etc.) | Gene expression, virus production, antibody production | HEK293, CHO, COS, stem cells | Sensitive Cells & maximum cell viability | High efficiency in stem cells, low cytotoxicity |
| AffiGEN® TransfectTM DNA In Vitro 2000 V2 | Wide range, including hard-to-transfect mammalian cells | Gene expression, virus production, protein production | Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) | Common cell types | Efficient for virus production, compatible with wide range of media |
| AffiGEN® TransfectTM DNA In Vitro 2000 Plus | Wide range, including hard-to-transfect mammalian cells | Gene expression, protein production | Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) | Very long DNAs | Improved efficiency and viability compared to AffiGEN® TransfectTM DNA In Vitro 2000 V2, reduced cytotoxicity |
| AffiGEN® TransfectTM DNA In Vitro 3000 | Wide range, including hard-to-transfect mammalian cells | Gene expression, virus production, protein production | Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) | Lentivirus, antibody & protein production | High efficiency with serum/antibiotics, compatible with wide range of media |
| AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit | Wide range, including hard-to-transfect mammalian cells | Gene expression, protein production | Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) | Best transfection with least toxicity | Biodegradable, compatible with wide range of media, low cytotoxicity |
| AffiGEN® TransfectTM DNA In Vitro 5000 | Wide range, including hard-to-transfect mammalian cells | Gene expression, virus production, protein production | Wide range, including hard-to-transfect cells (COS-7, HeLa, HepG2, etc.) | Common mammalian cells | High in-vivo delivery efficiency, compatible with wide range of media |