AffiCHECK® Enterovirus D68 (EV-D68) RNA - PCR Qualitative Positive Control
Product Datasheet - AFG-CHK-0488
PRODUCT DESCRIPTION:
AffiCHECK® Enterovirus D68 (EV-D68) RNA - PCR Qualitative Control is a high-quality, reliable standard containing known quantities of Enterovirus D68 (EV-D68) RNA. It's designed using modified and non-infectious parasite to ensure stability, thereby contributing to accurate and reliable results in PCR applications.
INTENDED USE:
AffiCHECK® Enterovirus D68 (EV-D68) RNA - PCR Qualitative Control serves as a comprehensive process control for the evaluation of the performance of nucleic acid tests for determination of the presence of Enterovirus D68 (EV-D68) RNA nucleic acid. It's essential in validating the performance of PCR assays in clinical, research, and diagnostic settings.
PRODUCT FEATURES:
- Specificity: Tailored for Enterovirus D68 (EV-D68) RNA detection.
- Formulation: Lyophilized for enhanced stability.
- Convenience: Ready-to-use upon reconstitution.
- Compatibility: Suitable for a wide range of PCR assays.
CHARACTERISTICS:
- Cultivation & Extraction: Cultivated in Vero cells and extracted using a reliable genomic RNA method.
- Quality Control: Subjected to rigorous testing to ensure batch consistency and reliability.
- Packaging: Provided in a protective, heat-sealed foil pouch with silica gel.
PERFORMANCE CHARACTERISTICS:
- Sensitivity: Optimized for high sensitivity in PCR detection.
- Specificity: Exclusively detects Enterovirus D68 (EV-D68) RNA.
- Reproducibility: Consistent results across different test runs and batches.
PACK CONTENT :
| Components | Pack Size | Note |
| RNA Control Lyophilized Powder (1.25x104 to 2.00x104 Copies/µL) | 1 Vial | Quantified by Real-Time PCR. |
| Buffer | 500 µL | Water, DNase, RNase Free. |
PRECAUTIONS:
- For research use or diagnostic control purposes only.
- Follow standard laboratory practices and use PPE.
- Use Sterile Filter Tips to prevent contamination.
- Handle infectious specimens with precautions.
- Disinfect work surfaces.
- Maintain separate working areas for testing.
- Dispose of unused reagents and waste as per regulations.
- May contain genetic material, handle with care.
- No method ensures total absence of infectious agents.
- Follow local waste disposal rules.
- Dilute below 103 copies/µL immediately before use.
- Avoid freezing for low-copy dilutions to prevent RNA degradation.
INSTRUCTIONS FOR USE:
- Open the foil pouch of AffiCHECK® Enterovirus D68 (EV-D68) RNA.
- Centrifuge AffiCHECK® Enterovirus D68 (EV-D68) RNA Lyophilized Powder at 1000 g for 1 minute.
- Add 100 μL of the Buffer and thoroughly mix until fully reconstituted.
- The concentration will be 1.25x104 to 2.00x104 Copies/µL once reconstituted.
- Vortex for 1 min for complete dissolution and homogenization.
RECOMMENDED STORAGE:
- Before Reconstitution: Store at 2-8ºC.
- After Reconstitution: Store at -20ºC.
- Shipping: No special instructions
- Shelf Life: 12 months from production date.
ORDERING INFORMATION :
| Catalog Number | Description | Size |
| AFG-CHK-0488 | AffiCHECK® Enterovirus D68 (EV-D68) RNA PCR Qualitative Positive Control | 1 vial + 500 µL Buffer |