AffiNGS® Ribo-off rRNA depletion kit (Plant)
The AffiNGS® Ribo-off rRNA depletion kit (Plant) is designed to remove ribosomal RNA (rRNA) from the total RNA of plant roots, seeds, and leaves. This kit is suitable for removing rRNA from plant total RNA with an initial template amount of 1 - 5 µg. The total RNA sample undergoes probe hybridization, RNase H digestion, DNase I digestion, and other steps for the rRNAs (including 5S, 18S, and 25S rRNA) to be depletes while retaining mRNA and other non-coding RNA, which can be user for the analysis of IncRNA and other non-coding RNA. The kit additionally provides digestion probes targeting mitochondrial rRNA and chloroplast rRNA, enabling it to efficiently remove major rRNAs from a variety of plant species and provide the most informa tive sequencing results. The kit is applicable for both intact and partially degraded RNA samples, and the resulting products are suitable for RNA library preparation and other experimental uses.
Components:
| Components | AFG-VZ-N409-01 (12 rxns) | AFG-VZ-N409-02 (24 rxns) |
| rRNA Probe (Plant) | 24 µl | 48 µl |
| Probe Buffer | 36 µl | 72 µl |
| Rnase H Buffer | 48 µl | 96 µl |
| Rnase H | 12 µl | 24 µl |
| DNase I Buffer | 348 µl | 696 µl |
| DNase I | 12 µl | 24 µl |
| Nuclease-free ddH2O | 1 ml | 2 x 1 ml |
↑ The colors of tube for each kit component are indicated.
Storage:
Store at -30 ~ -15°C and transport at ≤ 0°C.