AffiVET® Canine Brucella Antibody Rapid Test Kit

AffiVET® Canine Brucella Antibody Rapid Test Kit

• Species: Canine
• Disease or Pathogen: Brucellosis
• Target: Antibody
• Test Type: Rapid Tests

User Manual - Download Link

Kit Components:

• 10 Test Cassettes
• 10 Assay Diluents
• 10 Disposable Pipettes
• 1 Instruction Manual

Storage Conditions:

• Store the test kit at 2~30°C.
• DO NOT FREEZE.
• Avoid storing the test kit in direct sunlight.
• The test kit remains stable until the expiration date indicated on the package label.

Test Precautions:

• This kit is intended for brucellosis antibody detection in serum, plasma & whole blood. Ensure you fully understand its intended use.
• Carefully inspect the kit package and read the instructions to confirm the kit's validity and intact packaging. Only use valid kits.
• If any damage or leakage is detected, contact your vendor for a replacement. Do not use the kit unless otherwise agreed by the vendor.
• Handle all collected samples carefully, as they may be contagious, following local laws and regulations.
• Used test kits and tested samples should be handled carefully to avoid further contamination.
• Use the test cassette within 5 minutes after opening the foil package. Do not open without a planned experiment.
• Do not use components from other sources, as this will invalidate the results.
• The kit is designed for single use; do not reuse it.

Sample Preparation:

Use Canine whole blood, Serum or plasma.

• For Serum: Collect whole blood in a tube without anticoagulant. Let it sit at room temperature for 30 minutes, then centrifuge to separate the serum.
• For Plasma: Collect whole blood in a tube with anticoagulant, then centrifuge to obtain the plasma.

Test Procedure:

1. All reagents and samples must be at room temperature (15~30°C) before use.
2. Remove the test device from the foil pouch, and place it on a flat and dry surface.
3. Collect the sample using the disposable pipette and add 1 drop into the sample well of the test cassette.
4. Add 3 drops of the assay diluent into the sample well vertically.
5. Read the results after 15 minutes.

Note: Results observed after 15 minutes will be invalid.

Result Interpretation:

Negative Result:

• Presence of C-Line
• Absence of T-Line

Positive Result:

• Presence of C-Line
• Presence of T-Line

Invalid Result:

• Absence of C-Line
• Presence of T-Line

or

• Absence of C-Line
• Absence of T-Line

Test Limitations:

• The kit is designed for screening purposes and on-site testing.
• A positive result from this kit must be further confirmed by PCR and/or other official methodologies.
• Veterinarians should consider the clinical symptoms and laboratory results to make the final diagnosis.