AffiPCR® Taq DNA Polymerase
AffiPCR® Taq DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Champagne Taq DNA polymerase is still blocked at temperature up to 55°C which greatly prevents unspecific amplification. The DNA polymerase activity will be fully released at 95°C for 30 sec, which ensures excellent sensitivity and specificity. The activation of Champagne Taq DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). The 3' end of the PCR products is A and the PCR products can be cloned into T-vector. It is suitable for ClonExpress and TOPO cloning kit (AffiGEN #AFG-VZ-C112/AFG-VZ-C113/AFG-VZ-C115/AFG-VZ-C601).