AffiPCR® 2X Max Master Mix
Get the most out of your PCR experiments with AffiPCR® 2X Max Master Mix This powerful master mix includes two tubes of AffiPCR® Max reagents, specifically designed to provide maximum sensitivity and specificity in PCR. Our specialized formulation increases primer annealing efficiency, reducing the amount of unsuccessful experiments. Get ready for more successful PCR results with AffiPCR® Max Master Mix.
AffiPCR® 2X Max Master Mixis an upgraded versionMax Master Mix. Compared with the previous generation,it is the unique elongation factor, specificity-enhancing factor and plateau phase anti-inhibitor factor, which greatly improves the long-fragment amplification ability, amplification specificity and amplification yield. AffiPCR® Max can efficiently amplify up to 40 kb simple templates (e.g. λDNA, plasmids), 20 kb complex templates (e.g. genomic DNA) and 10 kb cDNA. The amplification error rate of AffiPCR® Max is 128-fold lower than that of conventional Taq DNA Polymerase. In addition, AffiPCR® Max has a good resistance to PCR inhibitors and can be used for direct PCR amplification of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. AffiPCR® Max contains two monoclonal antibodies inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. This product contains AffiPCR® Max Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. It only needs to add primers and templates to perform amplification, thereby reducing pipetting operations and improving detection throughput and reproducibility of results. The system contains protective agents that keep 2X AffiPCR® Max Master Mix stable in activity after repeated freezing and thawing. Amplification products are blunt-ended, which are compatible with ClonExpress kits.
Component:
Components | AFG-VZ-P515-01 | AFG-VZ-P515-02 | AFG-VZ-P515-03 |
2 X Max Master Mix | 1 mL | 5 × 1 mL | 15 × 1 mL |
Equivalence:
AFG-VZ-P505 | AffiGEN AffiPCR® Max Super-Fidelity DNA Polymerase |
AFG-VZ-515 | AffiGEN AffiPCR® 2X Max Master Mix |
AFG-VZ-525 | AffiGEN AffiPCR® 2X Max Master Mix (Dye Plus) |
F537S | Thermo Fisher Phusion Green Hot Start II High-Fidelity DNA Polymerase |
KOD-401 | TOYOBO KOD-Plus-Neo |
KFX-201 | TOYOBO KOD FX Neo |
M0493S | NEB Q5® Hot Start High-Fidelity DNA Polymerase |
M0494S | NEB Q5® Hot Start High-Fidelity 2X Master Mix |
R050A | TaKaRa PrimerSTAR® GXL DNA Polymerase |
R045A | TaKaRa PrimerSTAR® Max DNA Polymerase |
RR371A | Takara Ex Premier Polymerase Dye plus |
12351010 | Invitrogen Platinum™ SuperFi™ DNA Polymerase |
12344024 | Invitrogen AccuPrime™ Pfx DNA Polymerase |