AffiPCR® 2X Flash Master Mix (Dye Plus)

AffiPCR® 2X Flash Master Mix (Dye Plus)

This AffiPCR® 2X Flash Master Mix (Dye Plus) provides the perfect solution for your real-time PCR needs. This powerful, sensitive master mix contains an optimally-formulated blend of components that make it ideal for accurate, reliable amplification and data analysis. Every mix comes with EvaGreen® dye, an enzyme blend, and complementary buffer components, so you can always trust in the performance of your PCR assays.

AffiPCR® 2X Flash Master Mix (Dye Plus) is a new generation superior enzyme based on Flash Super-Fidelity DNA Polymerase. Through directed optimization of DNA Polymerase, AffiPCR® 2X Flash Master Mix (Dye Plus) DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec/kb) while maintaining high fidelity and yield. The amplification error rate of AffiPCR® Flash Super-Fidelity DNA Polymerase is 81-fold lower than that of conventional Taq DNA Polymerase. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling it to perform hot start PCR with great specificity. It contains all required reaction components AffiPCR® Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. This product contains tracking dyes, so PCR products can be directly loaded for electrophoresis after the reaction. Amplification will generate blunt-ended products, which are compatible with ClonExpress kits  and TOPO cloning kit.

Storage Conditions:

Store at temperatures between -30°C and -15°C, and ensure transportation at ≤0°C. After thawing, they can be stored at 4°C for up to three months, maintaining their quality and efficacy.

Equivalence: